李天然 叶阳 杨静雯 肖凌勇 王雪蕊 闫超群 邵佳凯 刘存志

摘要：目的 觀察留针与不留针情况下针刺“百会”配伍“足三里”对血管性痴呆（VD）大鼠认知功能及大脑皮层神经元损伤的影响，探讨留针与否的针刺效应。方法 采用永久性双侧颈总动脉结扎方法建立VD大鼠模型。实验大鼠随机分为假手术组、模型组、留针组、不留针组，每组8只。针刺模型大鼠“百会”+“足三里”后，Morris水迷宫实验检测大鼠空间学习记忆能力，尼氏染色检测大鼠大脑皮层神经元损伤。结果 与假手术组比较，模型组大鼠空间学习记忆能力明显降低，皮层神经元损伤明显；与模型组比较，留针组、不留针组空间学习记忆能力均显著提高，皮层神经元损伤改善；其中，留针组空间学习记忆能力提高更多，皮层神经元损伤改善更明显。结论 针刺可改善VD大鼠空间学习记忆能力和皮层神经元损伤，留针效果更好。

关键词：血管性痴呆；针刺效应；留针；认知功能；皮层神经元；大鼠

DOI：10.3969/j.issn.1005-5304.2018.05.014

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）05-0061-05

Effects of Acupuncture Needle Retaining or Not on Cognitive Function and

Cortical Neurons in Rats with Vascular Dementia

LI Tian-ran1，2， YE Yang2， YANG Jing-wen2， XIAO Ling-yong2，

WANG Xue-rui2，3， YAN Chao-qun2， SHAO Jia-kai2， LIU Cun-zhi4

1. Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China； 2. Acupuncture Center Affiliated to Beijing Hospital of TCM Affiliated to Capital Medical University， Beijing 100010， China； 3. Beijing Institute of Traditional Chinese Medicine， Beijing 100010， China； 4. Dongfang Hospital， Beijing University of

Chinese Medicine， Beijing 100078， China

Abstract： Objective To compare the effects of acupuncture needle retaining or not in “Baihui” compatibility “Zusanli” on cognitive function and cortical neurons in rats with vascular dementia （VD）； To discuss the acupuncture effects of acupuncture needle retaining or not. Methods VD model was established by permanent bilateral common carotid arteries occlusion. The experimental rats were randomly divided into sham-operation group， model group， needle retaining group， without needle retaining group， with 8 rats in each group. The acupoints “Baihui” and “Zusanli” were used. Morris water maze test was employed to detect the abilities of spatial learning and memory in rats. Nissls staining and immunofluorescence technique were used to observe the status of cortical neurons. Results Compared with sham-operation group， the abilities of spatial learning and memory decreased significantly and the injuries of cortical neurons were severe. Compared with model group， the abilities of spatial learning and memory in needle retaining group and without needle retaining group increased significantly and the injuries of cortical neurons were improved， among which， the abilities of spatial learning and memory was increased more， and the injuries of cortical neurons were better improved in the needle retaining group. Conclusion Acupuncture can improve the abilities of spatial learning and memory and cognitive function， and acupuncture needle retaining has better effects.

Keywords： vascular dementia； acupuncture effects； needle retaining； cognitive function； cortical neurons； rats

基金项目：北京市教育委员会科技计划重点项目（KZ201710025025）

通讯作者：刘存志，E-mail：lcz623780@126.com

血管性痴呆（vascular dementia，VD）是指以缺血缺氧性或出血性脑损伤造成的记忆、认知及行为等脑区低灌注的脑血管疾病所致的严重认知功能障碍综合征。我国65岁以上老年人VD患病率为1.2%～4.2%，70岁以上老年人年发病率为每千人6～12例[1]。与学习记忆有关的脑内结构主要是大脑皮质联合区、海马及其邻近结构。其中，大脑皮层特别是额叶、颞叶是远事记忆信息转变、贮存的主要部位[2]。……