王玲玲陈思马俊许巍岳涛赵振向王剑锋王晓棠刘艺鸣

帕金森病患者外周血单核细胞CD14和TLR4中mRNA的表达水平☆

王玲玲*陈思*马俊*许巍*岳涛*赵振向*王剑锋*王晓棠*刘艺鸣*

目的 研究帕金森病患者外周血单核细胞（peripheral blood mononuclear cell，PBMC）脂多糖受体（cluster of differentiation 14，CD14）和toll样受体4（toll-like receptor 4，TLR4）中mRNA的表达并探讨其在疾病发生发展中的临床意义。方法本实验共入组44例帕金森病患者和37例性别、年龄相匹配的健康对照者，并详细记录入组的44例帕金森病患者发病年龄、病程、性别，在关期时对帕金森病患者进行H-Y分期、UPDRSⅡ、UPDRSⅢ、NMSS评分，利用RT-PCR技术检测各研究对象PBMC中CD14和TLR4中mRNA的表达。结果 帕金森病患者外周血CD14 mRNA表达水平（1.459±0.658）2-△△CT、TLR4 mRNA（1.408±0.698）2-△△CT表达较健康对照者（（1.162±0.631）2-△△CT、（1.122±0.557）2-△△CT）均显著增加（P均＜0.05）。相关性分析显示帕金森病组CD14 mRNA的表达水平与反应帕金森病疾病严重程度的H-Y分期呈显著正相关（P＜0.05）。结论帕金森病患者外周血CD14 mRNA表达与其H-Y分期正相关，提示CD14/TLR4的单核细胞所介导的固有免疫反应可能是帕金森病发生、发展的一个重要因素。

帕金森病单核细胞信使RNA TLR4

【Abstract】Objective To investigate the expression levels of the CD14 mRNA and TLR4 mRNA in peripheral blood mononuclear cells from patients with Parkinson disease and to explore the clinical signicance.Methods Forty-four patients with Parkinson disease and 37 healthy controls were recruited.We recorded age of onset，duration of illness and sex of all recruited patients.PD patients were evaulated using the Hoehn-Yahr stages，Unified Parkinson Disease Rating Scale（UPDRS）Ⅱand UPDRSⅢ，non-motor symptoms scale（NMSS）on“off”time.Reverse transcription-polymerase chain reaction was performed to determine the expression levels of CD14 mRNA and toll-like receptor 4 （TLR4）mRNA.Results The expression levels of CD14 mRNA（1.459±0.658）2-△△CTand TLR4 mRNA（1.408±0.698）2-△△CTwas significantly up-regulated（P＜0.05）in Parkinson disease compared with controls（1.162±0.631）2-△△CT、（1.122± 0.557）2-△△CT）.In addition，there was positive correlation between the expression levels of CD14 mRNA in Parkinson disease patients with the Hoehn-Yahr stages.Meanwhile，there was no significant correlation between the expression levels of CD14 mRNA and TLR4 mRNA with other clinical scores.Conclusions There is positive correlation between the expression levels of CD14 mRNA in Parkinson disease patients with the Hoehn-Yahr stages，indicating that CD14/TLR4positive monocyte may be involved in the pathogenesis of the Parkinson disease.

【Key words】Parkinson disease Monocytes RNA Messenger TLR4

帕金森病（Parkinson disease，PD）是常见的神经系统变性疾病［1］，临床表现除典型的运动症状外，常伴有便秘、抑郁、感觉异常等非运动症状，其发病机制至今仍不十分明确。PD的病因很多，包括遗传因素、环境因素、年龄老化、氧化应激、免疫炎症等，免疫炎症因素近来受到更多的关注。

尽管目前对PD的中枢免疫炎性机制的研究较多［2-4］，但关于PD的外周固有免疫的研究较少。单核细胞是外周固有免疫应答的重要组成部分，本实验旨在探讨PD外周血单核细胞中CD14和TLR4mRNA的表达水平及其与PD的临床评分的相关性，并探讨免疫异常在PD发生、发展中的作用。……