孙志清付庆喜涂丽香车峰远苏全平

耐药癫痫患者和难治性癫痫大鼠脑组织与外周血GST-π蛋白表达☆

孙志清*付庆喜*涂丽香*车峰远*苏全平*

目的 考察耐药癫痫患者和难治性癫痫大鼠脑组织与外周血谷胱甘肽S-转移酶π（glutathione S-transferase π，GST-π）的表达。方法 临床收集2010年1月至2014年3月在我院神经外科行手术治疗的耐药性癫痫患者32例，选择同期因脑血管畸形手术切除的10例正常脑组织为对照；动物试验：收集15只对照组和20只难治性癫痫大鼠组，难治性癫痫依据锂-匹罗卡品癫痫模型制备。检测人与大鼠外周血和脑的GST-π水平表达，并进行组间比较。结果 耐药癫痫患者脑组织的颞叶20例，额叶6例，枕叶6例。GST-π在耐药癫痫患者和难治性癫痫大鼠脑的颞叶、额叶、枕叶中均有表达，存在于细胞胞浆，染成浅黄色至棕黄色颗粒，与对照组比，耐药癫痫患者和难治性癫痫大鼠脑和外周血GST-π表达明显增加（P＜0.05）。结论 GST-π可能参与耐药癫痫的发病，检测外周血GST-π为耐药性癫痫的治疗提供参考。

GST-π耐药癫痫 难治性癫痫大鼠

【Abstract】Objective To investigate the expression of glutathione S-transferase π（Glutathione S-transferase π，GST-π）protein in peripheral blood and brain of patients with drug-resistant epilepsy and refractory epilepsy rats.Methods From January 2010 to March 2014，the expression of GST-π in the blood and brain of 32 cases of drug-resistant epilepsy underwent neurosurgery and 10 cases of cerebral vascular malformation underwent surgery were studied and compared.The expression of GST-π in the blood and brain in refractory epilepsy rats and normal rats were studied and compared.Results The specimen from 20 temporal，6 frontal and 6 occipital lobes were obtained from drug-resistant epilepsy patients.The expression levels of GST-π in the blood and brain in refractory epilepsy rats and normal rats were higher than those of the control groups（P＜0.05）.Conclusion GST-π may be involved in the process of drug-resistant epilepsy. The GST-π expression in blood may be used as a marker for resistance to anti-epileptic agents.

【Key Words】GST-π Protein Drug-Resistant Epilepsy Refractory Epilepsy

癫痫是中枢神经系统常见的发作性疾病，以短暂性、反复性和刻板性为发病特征，临床中超过1/4的癫痫患者发展为耐药性癫痫（drug resistant epilepsy），或称为难治性癫痫（refractory epilepsy），严重影响患者的生活质量［1］。耐药性癫痫成为临床治疗中的难点，耐药性是导致临床上癫痫治疗失败的重要原因，耐药的发生是复杂的生物过程。由于抗癫痫药与肿瘤耐药基因作用的底物相似，肿瘤的耐药基因可能也参与了耐药癫痫。……