郑克彬解靖李艳萌史帅史彦芳

脑胶质瘤环磷腺苷效应元件结合蛋白的表达

郑克彬*解靖△李艳萌△史帅△史彦芳*

目的 探讨环磷腺苷效应元件结合蛋白（cAMP-response element binding protein，CREB）在不同级别脑胶质瘤组织中的表达及其临床意义。方法 采用免疫组化法检测CREB在5例颅脑损伤减压术后脑组织切片，55例不同分化程度脑胶质瘤组织切片中的表达，并采用Western blot、RT-PCR检测CREB在10例颅脑损伤减压术后脑组织标本，30例不同分化程度脑胶质瘤组织中的表达。结果 免疫组化法测得CREB的阳性表达率分别为对照组2/5，Ⅰ-Ⅱ级胶质瘤10/15，Ⅲ级胶质瘤11/12，Ⅳ级胶质瘤28/28，免疫组化显示数据总体上有统计学差异（H=28.183，P＜0.05）。RT-PCR法检测2-ΔΔCt值，对照组：1.00±0.00，Ⅰ-Ⅱ级胶质瘤：1.35±0.07，Ⅲ级胶质瘤：2.88±0.11，Ⅳ级胶质瘤：3.75±0.20。脑胶质瘤组织中CREB的表达均高于对照组脑组织，且各级别癌组织间有统计学意义（F=1.208，P＜0.05）。Western blot法测得对照组：0.311±0.014，Ⅰ-Ⅱ级胶质瘤：0.469±0.026，Ⅲ级胶质瘤：0.641±0.028，Ⅳ级胶质瘤：0.896±0.024，各组间有统计学差异（F=1.123，P＜0.05）。结论 CREB在不同分化程度脑胶质瘤组织中均存在过表达现象，与肿瘤的分化程度呈正相关，可能在胶质瘤发生发展过程中起着重要的调节作用。

脑胶质瘤 CREB过表达

【Abstract】Objective To investigate the clinical significance and abnormal expression of the CREB in different grade gliomas.Methods The expression of CREB was examined by using immunohistochemistry in brain tissues from the brain injury（5 cases）and different grade gliomas（55 cases）.The mRNA and protein levels of CREB were further assessed using Western blot and RT-PCR in brain tissues from the patients with brain injury（10 cases）and those with different grade gliomas（30 cases）.Results The positive rates of CREB immunohistochemistry were 2/5 in control，10/15 in Ⅰ-，Ⅱ11/12 inⅢ，28/28 inⅣ.The positive rates of CREB were significantly different among different groups（H= 28.183，P＜0.05）.The mRNA levels of CREB were 1.00±0.000 in control，1.35±0.068 inⅠ-Ⅱ，2.88±0.111 inⅢand 3.75±0.196 inⅣ.The expression of CREB was higher in the glioma than in control group，and the mRNA levels of CREB were significantly different among different groups（F=1.208，P＜0.05）.The protein levels of CREB were 0.311± 0.014 in control，0.469±0.026 inⅠ-Ⅱ，0.641±0.028 inⅢand 0.896±0.024 inⅣ.The protein levels of CREB were significantly different among different groups（F=1.123，P＜0.05）.Conclusion The expression of CREB is elevated in gliomas with different differentiation degrees.The expression of CREB was positively correlated with the degree of differentiation，indicating that CREB may have an important regulatory role in the progress of gliomas.

【Key words】Glioma CREB Over-expression

胶质瘤目前是神经外科领域常见的恶性肿瘤之一，它是发生于神经外胚层，由间质细胞及实质细胞形成的肿瘤。近年来有关资料显示CREB高表达与多种类型肿瘤的发生和发展密切相关。本研究通过免疫组化法（Immunohistochemistry，IHC）、蛋白质印记法（Western blot，WB）和逆转录-聚合酶链反应（RealtimePCR，RT-PCR）的方法在组织水平、基因水平以及蛋白水平探究目的蛋白CREB在不同脑组织的表达情况，并通过统计学分析CREB与脑胶质瘤患者临床病理特征之间的关系，进而了解CREB在脑胶质瘤中的临床意义，并为后续实验研究提供理论依据。……