安淼 余贤美 王洁 刘加芬 艾呈祥

摘 要：为建立一种同时测定6种柿叶黄酮类物质的方法，通过对液相色谱检测波长、流动相、柱温等条件的探索，最终确定了芦丁、山柰素、异鼠李素、槲皮素、异槲皮苷和紫云英苷6种黄酮类物质标准品的液相色谱检测条件：以十八烷基硅烷键合硅胶为填充剂，以甲醇为流动相A，以0.5%乙酸溶液为流动相B，进行梯度洗脱，0～100 min A∶B为20∶80，105 min后为40∶60；检测波长为360 nm；柱温为35℃；体积流量1.0 mL/min。并以两个品种的柿叶为样品，对所建立的方法进行验证，得到了预期结果，说明建立的检测方法是可行的。

关键词：柿叶；黄酮类物质；高效液相色谱；同时检测

中图分类号：S665.209.9文献标识号：A文章编号：1001-4942（2016）05-0131-06

Abstract The HPLC method for simultaneous detection of 6 flavonoids from persimmon leaves was established by exploring the detection conditions such as wavelength， mobile phase and column temperature. The 6 flavonoids were lutin， kaempferol， isorhamnetin， quercetin， isoquercitrin and astragalin. The results showed that the 6 flavonoid standards could be detected simultaneously under the following conditions： using C18 as stuffing bulking agent， carbinol as mobile phase A and 0.5% acetic acid as mobile phase B； the samples were gradiently eluted at the ratios of phase A∶B as 20∶80 in 0～100 minutes and 40∶60 after 105 minutes； and the detection was carried out with 360 nm of wavelength， 35℃ of column temperature and 1.0 mL/min of volume flow rate. The detection method was verified with the leaves of two persimmon varieties， and the expected results were obtained， which indicated that the established method was feasible.

Key words Persimmon leaves； Flavonoid； HPLC； Simultaneous detection

柿叶中含有丰富的黄酮苷、芦丁、三萜类、酚类、胆碱、维生素C、胡萝卜素、香豆素、植物甾醇类、有机酸、脂肪酸、挥发油、生物碱、还原糖、多糖、鞣质、香脂精、多种氨基酸及铁、锌、钙等对人体健康有益的营养成分[1]。近几年，随着人们越来越注重健康的生活，柿叶的功能成分逐渐受到重视。

黄酮类物质是柿叶中最主要的功能成分，已发现的柿叶黄酮类物质主要有紫云英苷、黄芪苷、槲皮素、异槲皮苷、山柰酚、芦丁、芸香苷、金丝桃苷、杨梅树皮苷等[2]。对柿叶黄酮的开发研究，可提高柿树的利用效率，增加其经济效益。

目前，对于柿叶黄酮的研究主要是总黄酮或少量几种黄酮类物质的提取[3～5]、检测方法及含量分析[6，7]，以及不同品种间总黄酮含量的比较[8]。关于同时检测柿叶中6种黄酮类物质的方法迄今未见报道。……