温晓薇，周丽霞，解军，匡延平，柴蔚然



·综述·

人卵泡液蛋白质组学的研究进展

温晓薇，周丽霞，解军，匡延平，柴蔚然△

卵泡液是由卵母细胞、颗粒细胞和膜细胞等共同分泌的一种含多种蛋白质的生物液，其作为卵母细胞生长的微环境或培养基，直接影响到卵泡的发育和卵母细胞的成熟。过去十几年，对卵泡液蛋白质组学的研究主要集中于新蛋白筛选及疾病相关蛋白等的识别上。本文从卵泡液新蛋白的筛选、年龄相关生物学标志鉴定、确定卵泡成熟标志、疾病相关标志的筛选和体外受精（IVF）成功的标志等5方面介绍近年卵泡液蛋白质组学的相关研究。筛选和鉴定某些生殖和发育相关的关键蛋白，并可能成为卵泡液中的生物标志，可用于预测卵母细胞的质量、受精及胚胎发育潜能。

卵泡液；蛋白质组学；生物学标记；受精，体外

【Abstract】Follicular fluid is a biological fluid containing a varity of the proteins and factors secreted by oocytes，granulosa cells and theca cells，which is important for the follicular development and oocytematuration as the medium or micro-environment of oocyte growth.Over the past ten years，the proteomic studies of follicular fluid were focused on the identification of some novel proteins and screening of disease-related proteins.In this article，five aspects related to the proteomic studies of follicular fluid were reviewed as follows:some new proteins identified from follicular fluid，the age-related biomarkers，some definite biomarkers of follicular maturation，the disease-related markers，and the biomarkers related to IVF success.It is possible that the quality of follicle and oocyte，and the potential of fertilization and embryo development，be predicted by some potential biomarkers of follicular fluid that are the key reproduction-related and（or）developemnt-related proteins screened and identified from those proteomic studies.

【Keywords】Follicular fluid；Proteomics；Biologicalmarkers；Fertilization in vitro

（JIntReprod Health/Fam Plan，2016，35：303-307）

卵泡液是人体内由卵母细胞产生并包含多种蛋白质的一种重要分泌物，其作为卵母细胞生长的培养基或微环境，对卵泡发育和卵母细胞的成熟发挥着重要的作用。在卵泡形成过程中，原始卵泡发育为初级生长卵泡，初级生长卵泡再发育为次级生长卵泡，原始卵泡、初级生长卵泡和次级生长卵泡也称为窦卵泡前期。在卵泡周围包围着数层颗粒细胞，颗粒细胞会蓄积卵泡液，而卵泡液会随着卵母细胞的增大而不断增加。一般认为，卵泡液是由于卵泡在形成过程中对血浆蛋白通透性不断增加，使得血浆蛋白跨过血-卵屏障与卵泡周围的颗粒细胞、卵母细胞及卵泡膜细胞分泌物共同构成的。……