运脾方药对幼龄营养性肥胖大鼠瘦素胰岛素影响的实验研究

职冬丽1，王明明2*

(南京中医药大学第一临床医学院，南京 210029 )

摘要：目的观察苍术山楂颗粒对高脂饮食诱导的幼龄营养性肥胖大鼠体质量、血清瘦素及胰岛素水平的影响。方法采用高脂饮食喂养刚离乳(3周龄)SD大鼠,诱导幼龄营养性肥胖大鼠模型，4周后予苍术山楂颗粒灌胃治疗,观察幼龄营养性肥胖模型大鼠体质量、瘦素及胰岛素的变化。结果苍术山楂颗粒灌胃幼龄营养性肥胖大鼠4周后,治疗组大鼠的体质量(375.50±13.19) g明显低于模型组(404.10±2.59) g，2组比较，差异有统计学意义，P<0.01；治疗组大鼠的血清瘦素(2.51±0.38) ng/mL较模型组(3.37±0.21) ng/mL有所下降，2组比较，差异有统计学意义，P<0.05;治疗组大鼠的血清胰岛素(13.27±0.60) mIU/L明显低于模型组(18.39±0.82) mIU/L,2组比较，差异有统计学意义，P<0.01。结论苍术山楂颗粒通过降低幼龄营养性肥胖大鼠体内较高的瘦素及胰岛素水平来达到减重的目的。

关键词：运脾方药；幼龄营养性肥胖大鼠；瘦素；胰岛素

DOI:10.13463/j.cnki.jlzyy.2015.04.026

中图分类号：R725.8文献标志码： A

文章编号：1003-5699(2015)04-0396-03

基金项目:南京中医药大学中医儿科学重点学科开放课题资助项目(EZK2012017)。

作者简介:职冬丽(1982-)，女，硕士研究生，主治医师，主要从事小儿脾系疾病研究。

收稿日期:(责任编辑:赵玉芝2014-09-05)

*通信作者:王明明，电话-13512539114，电子信箱-mingmingwang330@126.com

Yun-pi Prescription for leptin and insulin influence on young nutritional obesity rats

ZHI Dongli1,WANG Mingming2*

(First Clinical Medical College of Nanjing University of Chinese Medicine,Nanjing 210029,China)

Abstract:ObjectiveObservation of rhizoma atractylodis haw granule on the young nutritional obesity rats induced by high-fat diet weight,blood thin element and the influence of insulin levels.MethodsHigh fat diet just from the milk of age (3 w) SD rats,induced the young in the rat model with nutritional obesity,4 weeks after receiving the payment rhizoma atractylodis hawthorn particles lavage treatment,observation of young alimentary obesity model rats changes in body weight,leptin and insulin.ResultsRhizoma atractylodis hawthorn particles lavage young nutritional obesity rats after 4 weeks,the treatment group rats body quality (375.50±13.19) g significantly lower than the model group (404.10±2.59) g,comparing the two groups,the difference was statistically significant,P<0.01;The treatment group rats blood thin element (2.51±0.38) ng/mL compared with model group (3.37±0.21) ng/mL decreased,the difference was statistically significant,P<0.05;The treatment group rats serum insulin (13.27±0.60) mIU/L significantly lower than the model group (18.39±0.82) mIU/L,comparing the two groups,the difference was statistically significant,P<0.01.ConclusionRhizoma atractylodis hawthorn particles by reducing juvenile nutritional obesity rats’ higher leptin and insulin levels to achieve the goal of weight loss.

Keywords:Yun-pi Prescription;young nutritional obesity rats;leptin;insulin

流行病学研究表明，肥胖儿童多数伴有瘦素抵抗和胰岛素抵抗。瘦素抵抗是指机体组织对瘦素的调节作用减弱或无反应，胰岛素抵抗是指机体组织对胰岛素的反应性降低或丧失。且肥胖儿童血清瘦素与胰岛素呈正相关关系，即肥胖儿童体内存在着高瘦素血症和高胰岛素血症[1]。中医认为，脾失健运、湿浊瘀阻是儿童单纯性肥胖症的主要病机，因此运脾化湿逐瘀是本病的主要治疗原则。前期的动物实验研究[2]表明，苍术山楂颗粒可以改善幼龄营养性肥胖大鼠异常的糖脂代谢，本研究拟观察苍术山楂颗粒对幼龄营养性肥胖大鼠体质量、瘦素及胰岛素的影响，以期进一步阐明运脾方药对幼龄营养性肥胖大鼠体内高瘦素及高胰岛素水平的调整作用。……