半枝莲总黄酮对反复脑缺血再灌注小鼠脑能量代谢的影响

马霄1，邵南齐1，陈晓敏1，苗明三2*

(1.郑州澍青医学高等专科学校，郑州 450064;2.河南中医学院，郑州 450008)

摘要：目的探讨半枝莲总黄酮对反复脑缺血再灌注小鼠脑能量代谢的影响。方法将小鼠随机分为6组，采用双侧颈总动脉夹闭,建立反复脑缺血再灌注致学习记忆障碍小鼠模型。测定LD及TchE、LDH、ATP活力。结果与模型组相比，半枝莲总黄酮中剂量组可显著提高小鼠脑TchE活力(P<0.01)；半枝莲总黄酮大、中剂量组均能显著、明显降低小鼠脑LD含量(P<0.01，P<0.05)，半枝莲总黄酮大、中剂量组均可显著、明显提高小鼠脑LDH活力(P<0.01,P<0.05)；半枝莲总黄酮大、中剂量组均能显著、明显提高小鼠脑Na+-K+-ATP、Ca2+-Mg2+-ATP、Ca2+-ATP酶活力(P<0.01，P<0.05)。结论半枝莲总黄酮对反复脑缺血再灌注小鼠引起的脑神经损伤具有保护作用，机制可能与其减轻脑水肿、改善脑细胞能量代谢有关。

关键词：半枝莲总黄酮；脑缺血再灌注；能量代谢；小鼠

DOI:10.13463/j.cnki.jlzyy.2015.04.027

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)04-0399-03

基金项目:河南省重大公益性利研计划(081100911000)；郑州澍青医学高等专科学校重点课题(12002)。

作者简介:马霄(1981-)，女，硕士研究生，讲师，主要从事药理学及中药药理学的教学与研究。

*通信作者:苗明三，电话-(0371)65362546，电子信箱-miaomingsan@163.com

Total flavonoids of scutellaria barbata on repeated cerebral ischemia

reperfusion for mice’s energy metabolism of brain

MA Xiao1,SHAO Nanqi1,CHEN Xiaomin1,MIAO Mingsan2*

(1.Zhengzhou Shuqing Medical College,Zhengzhou 450064,China;

2.Henan University of TCM,Zhengzhou 450008,China)

Abstract:ObjectiveTo explore the effect of total flavonoids of scutellaria barbata on repeated cerebral ischemia-reperfusion for energy metabolism of mice.MethodsThe mice were randomly divided into six groups.Using bilateral common carotid arteries clip to establish repeated cerebral ischemia reperfusion mice model with learning and memory disorder.Determinate the activity of LD and TchE,LDH,ATP.ResultsCompared with the model group,medium dose of total flavonoids of scutellaria barbata group can significantly improve the mouse TchE activity (P<0.01);Large and medium dose of total flavonoids of scutellaria barbata group can significantly and obviously reduce mouse cerebral LD content (P<0.01,P<0.05);Large and medium dose of total flavonoids of scutellaria barbata group can obviously improve the mouse LDH activity (P<0.01,P<0.05);Large and medium dose of total flavonoids of scutellaria barbata group could significantly increase the mouse Na+-K+-ATP,Ca2+-Mg2+-ATP,Ca2+-ATP enzyme activity (P<0.01,P<0.05).ConclusionTotal flavonoids of scutellaria barbata have protection effect of brain nerves injury which caused from repeated cerebral ischemia-reperfusion mice,its mechanism could have related to relieve cerebral edema and improve the energy metabolism of brain.

Keywords:total flavonoids of scutellaria barbata;repeated cerebral ischemia-reperfusion;energy metabolism;mice

脑缺血是临床常见的脑血管疾病之一[1]，局部的脑缺血障碍，会使脑缺血、缺氧，可造成脑细胞损伤，脑细胞缺血最易受损的是脑神经，而脑神经功能与脑能量代谢密切相关[2]。半枝莲为唇形科植物半枝莲Scutellaria barbata D.Don的干燥全草，半枝莲总黄酮是半枝莲的提取物，具有清热解毒、散瘀止血、活血止痛、利尿消肿、抗癌等功效[3-4]。本研究通过结扎双侧颈总动脉造反复脑缺血模型，旨在探讨半枝莲对小鼠脑能量代谢的影响。

1实验材料

半枝莲购自张仲景大药房，半枝莲总黄酮为郑州澍青医学高等专科学校药学实验室提供，采用醇提法提取，半枝莲总黄酮含量为60%；养血清脑颗粒(当归、川芎、白芍、熟地黄、钩藤、鸡血藤、夏枯草、决明子、珍珠母、延胡索、细辛等),天津天士力制药股份有限公司；……