针药结合疗法对帕金森病模型小鼠纹状体区多巴胺D1、D2受体活性的影响

陈洁1，姜超2，陆征宇1，张琦君1

摘要：目的探讨针药结合疗法对帕金森病(PD)模型小鼠纹状体区多巴胺D1、D2受体活性的影响。方法将C57B L/6雄性小鼠随机分为正常组、模型组、针刺组、中药组、西药组及针药结合治疗组，每组再随机分为7天组(n=7)和14天组(n=7)，应用腹腔内注射(MPTP)的方法制备PD模型小鼠。针刺组针刺百会、运动前区(双侧)、哑门；西药治疗组灌胃美多巴；中药组灌胃中药PD方；针药结合组针刺和中药同时施行，方法同中药组和针刺组。用免疫组化法观察治疗后PD模型小鼠脑内纹状体区多巴胺(DA)D1、D2受体活性的影响。结果针药结合组、西药治疗组小鼠脑内纹状体区DA的D1、D2受体活性与模型组比较，差异有统计学意义(P<0.01)。西药治疗PD模型小鼠，其模型小鼠纹状体区D1、D2受体活性改善最佳，最接近正常组D1、D2受体活性指标。针药结合治疗改善PD模型小鼠纹状体区D1、D2受体活性优于单纯中药治疗及针刺治疗。结论西药美多巴治疗PD，改善该病多巴胺D1、D2受体活性且具有明显优势，针药结合对PD的治疗优于传统单纯中药治疗及针刺治疗。

关键词：帕金森病；针药结合疗法；免疫组化；多巴胺；小鼠

中图分类号：R742R285

收稿日期：(2014-10-05)

Effects of Acupuncture Combined with Herbar Medichines on Striatal Dopamine D1 and D2 Receptor Activity in Mice with Parkinson’s Disease

Chen Jie，Jiang Chao，Lu Zhengyu，Zhang Qijun

Yueyang Hospital of Integrated Chinese and Western Medicine，Shanghai University of Traditional Chinese Medicine，Shanghai 200433，China

Abstract：ObjectiveTo investigate the effect of acupuncture combined with herbal medicine on striatal neurotransmitters dopamine (DA) of D1 and D2 receptor activity in mouse with Parkinson's disease(PD).MethodsTwenty-eight adult male C57B L/6J mice were randomly divided into normal control group，PD model group，acupuncture group，herbal medicine group，western drug group，and acupuncture and herb combination group.Each group was divided into 2 subgroups，the 7-day and 14-day(n=7).The PD models were established with intraperitoneal injections of MPTP in C57/BL mice.The mice in acupuncture group were applied to Baihui，section of premotor area(both sides)，Yamen The mice in western drug group were treated with madopa.The mice in herbal medicine group were treated with PD prescription.The mice in acupuncture and herb combination group were treat with both acupuncture and herbal medicine.The effect of striatal neurotransmitters DA of D1 and D2 receptor activity were measured by immunohistochemical method after treatment.ResultsThere were significant differences on striatal neurotransmitters DA of D1 and D2 receptor activity among the acupuncture combined with herbal medicine group，the western drug group and the normal control group(P<0.01).The striatal neurotransmitters DA of D1 and D2 receptor activity demonstrated a significant improvement in western drug group comparing to other groups.The striatal neurotransmitters DA of D1 and D2 receptor activity demonstrated a significant improvement in the acupuncture combined with herbal medicine group，which comparing to the acupuncture group and the herbal medicine group. ConciusionMadopa can effectively improve the striatal neurotransmitters DA of D1 and D2 receptor activity.The acupuncture and herb combination group was more significant than the acupuncture group and the herbal medicine group in improvement of the striatal neurotransmitters DA of D1 and D2 receptor activity.

Key words：Parkinson’s disease；acupuncture combined with herbal medicine；Mice

作者单位：1.上海中医药大学附属岳阳中西医结合医院(上海 200433)，E-mail：happycatl@126.com；2.陕西中医学院附属医院

帕金森病(PD)是一种以黑质多巴胺能神经元(NDN)特征性缺失，广泛累及中枢神经系统、症候复杂多样的慢性神经元变性疾病。目前，关于PD的研究表明其与遗传因素、环境因素、兴奋性毒性与钙的细胞毒作用、线粒体功能障碍与氧化应激过度、免疫学异常、年龄老化、细胞凋亡、泛素蛋白酶体系统功能障碍、多聚 ADP核糖聚合酶过度活化等诸多因素有关，然根本发病机制尚未完全阐明[1]。L-dopa制剂目前仍被认为是治疗PD的金标准，可明显改善PD症状，但50%～80%的PD患者在服药后平均4.1年的时间里，出现了运动并发症[2]。……