张旭升 朱平先 黄战军 蔡博治 张昕 张勇刚

摘要：目的在大鼠血管鈣化模型上，探讨麝香保心丸对血管钙化的影响及其可能作用机制。方法实验动物随机分正常组、钙化组及钙化+麝香保心丸组，每组6只。钙化组采用维生素D3（3×105 U/kg 1次，肌肉注射）和尼古丁（25 mg/kg溶于花生油中，早、晚各灌胃1次）诱导大鼠血管钙化模型，钙化+麝香保心丸组于造模后第2天开始，每天麝香保心丸灌胃[14.0 mg/（kg·d）]，用Von Kossa染色检测血管钙化程度，用钙离子测试盒、碱性磷酸酶（ALP）试剂盒测定大鼠主动脉钙含量和ALP活性，用放射免疫法检测大鼠血浆单核细胞趋化蛋白1（MCP1）含量，用免疫组织化学法检测血管组织MCP1受体表达。结果维生素D3和尼古丁能够诱导典型大鼠血管钙化模型，Von Kossa染色可见血管钙化模型大鼠主动脉有大量黑色颗粒沉淀，钙化组血管钙含量、ALP活性高于正常组（P<0.01），同时，血浆MCP浓度、血管组织MCP1及其受体表达明显上调（P<0.01）;用麝香保心丸干预后，血管钙化程度减轻（P<0.01）。血浆MCP1及其受体的表达下调，差异有统计学意义。结论麝香保心丸可抑制血管钙化，并且提示可能通过下调MCP1表达和ALP活性抑制血管钙化的发生和发展过程。

关键词：麝香保心丸;血管钙化; 单核细胞趋化蛋白1;大鼠

中图分类号：R288.5文献标识码：A

doi：10.3969/j.issn.16721349.2015.01.019

文章编号：16721349（2015）01005203

Effect of Shexiang Baoxin Pill on Vascular Calcification in Model Rats

Zhang Xusheng，Zhu Pingxian， Huang Zhanjun，Cai Bozhi， Zhang Xin， Zhang Yonggang

Longgang District Peoples Hospital of Shenzhen，Shenzhen 518172，Guangdong，China

Corresponding Author：Zhu Pingxian

Abstract：ObjectiveTo investigate the effect and possible mechanism of Shexiang Baoxin Pill（SBP） in aorta of vascular calcification model rats.MethodsSevenweekold male SpragueDawley （SD） rats were randomly divided into normal group， calcification group and calcification plus SBP group （all n=6）.On the first day in calcification and calcification plus SBP group， vascular calcification was induced by a single dose intramuscular injection of vitamin D3 （300 000 U/kg） in the morning plus two doses of nicotine （25 mg/kg dissolved in peanut oil ） gavage in the morning and in evening.The next day after calcification induction， rats in SBP group were gavaged with SBP[14 mg/（kg·d）] for four weeks.Vascular calcification was confirmed by Von Kossa staining.Calcium content and alkaline phosphatases （ALP） activity were detected by calcium assay kit and ALP detection kit respectively.The contents of plasma monocyte chemotactic protein1 （MCP1）  were determined by radioimmunoassay， and the expressions of corresponding receptors were determined by immunohistochemistry. ResultsVon Kossa staining showed that there were mass black granules deposited in aortic wall of the vascular calcified rats.Calcium content and ALP activity in calcified group were increased significantly than those in the control group （P<0.01）.Meanwhile， MCP1 contents in plasma and corresponding receptor level in calcified group were upregulated significantly compared with those in the control group（P<0.01）.In addition， SBP diet could reduce the degree of vascular calcium contents， ALP activity and MCP1 content.ConclusionSBP significantly protects the aorta against calcification partly through reducing MCP1 content and ALP activity.

Key words：Shexiang Baoxin pill;vascular calcification;monocyte chemotactic protein1;rat

血管钙化是目前临床上常见的病理表现，普遍存在于高血压、动脉粥样硬化、糖尿病和慢性肾功能不全等疾病，亦存在于老年人生理性钙化中，是导致心脑血管急性事件的重要危险因子。国内外研究表明，血管钙化与生理性骨质形成类似，是主动的、可调节的过程[1]。目前证实许多活性因子如心血管组织分泌的活性因子（内皮素、尾加压素Ⅱ等）[2，3]和醛固酮[4]等都参与了血管钙化的调节过程。本课题组近期发现[5]，炎症因子参与血管钙化的发生与发展，然而，血管钙化的机制非常复杂，具体机制尚未完全阐明，临床上寻找防治血管钙化的药物，是当前研究领域的重点。……