摘要[目的]运用电感耦合等离子體质谱法和原子荧光法对植物性食品中的硒含量进行测定。[方法]试验采用密闭高压消解技术和湿法消解对样品进行前处理，分别采用电感耦合等离子体质谱法和原子荧光法测定植物性食品中痕量硒的含量，并对2种方法进行了对比分析。[结果]研究表明，2种方法的线性回归方程的相关系数均大于0.999，其方法检出限分别为0.01、0.04 μg/L，加标回收率在95.6%～103.0%，相对标准偏差均小于5%，2种方法均具有良好的准确度和灵敏度。[结论]试验表明，电感耦合等离子体质谱法简单快速、线性范围宽，更加省时，效率更高。

关键词 电感耦合等离子体质谱法；原子荧光法；植物性食品；硒

中图分类号S609.9文献标识码

A文章编号0517-6611（2015）05-228-03

Comparative of Determination of Selenium in Plant Food by Inductively Coupled Plasma Mass Spectrometry and Atomic Fluorescence Spectrometry

JIANG Yong-hong （Guangxi Center for Disease Prevention and Control，Nanning，Guangxi 530028）

Abstract [Objective] Selenium content in plant food was determined by using Inductively Coupled Plasma Mass Spectrometry （ICP-MS） and Atomic Fluorescence Spectrometry （AFS）.[Method] The methods of Inductively Coupled Plasma Mass Spectrometry （ICP-MS） and Atomic Fluorescence Spectrometry （AFS） for determination of selenium in plant food were developed and compared.The samples were digested by sealed high pressure digestion and wet digestion.The good veracity and precision were obtained in the comparison study on the two methods.[Result] The correlation coefficients for the standard curves of two methods were greater than 0.999，and the detection limits were 0.01 and 0.04 μg/L respectively.The recoveries varied from 95.6% to 103.0%，and the relative standard deviations were less than 5%.[Conclusion] The experimental results show that ICP-MS is simple，rapid，and which has a wide linear range and the precision and recovery are better than AFS.

Key words Inductively Coupled Plasma Mass Spectrometry； Atomic Fluorescence Spectrometry； Plant food； Selenium

作者简介江永红（1968- ），女，广西融安人，主管技师，从事理化检验工作。

收稿日期2014-12-26

硒是人体必需的15种微量元素之一，参与体内许多重要的代谢过程，被称为生命元素。它能清除生物体内的活性氧自由基，提高机体免疫功能，有效防止心血管疾病、肿瘤、癌症等疾病的发生，但摄入过量的硒会导致体内的血红蛋白和谷丙转氨酶升高，血糖、谷胱甘肽降低，从而对人体造成伤害[1]。因而对食品中硒含量进行检测具有重要的意义。

目前测定硒的主要方法有：分光光度法[2-3]、原子吸收光谱法[4-6]、电化学法[7-8]、原子荧光法[9-10]和电感耦合等离子体质谱法[11-13]。国家标准中以微波消解氢化物-原子荧光法测定蔬菜中的硒，胡兰基等用微波消解法对样品进行前处理使用原子荧光法对鲜黄蘑菇中的硒含量进行了测定[14]，但该方法对酸度和温度要求较为苛刻，操作繁琐。……