李进波 夏明元 戚华雄

摘要

[目的] 获得水稻抗稻瘟病基因Pi1和Pi2聚合系，并对其进行抗性评价。[方法]

以GD7为稻瘟病抗性基因供体，扬稻6号为受体，通过回交转育结合分子标记辅助选择，选育出10个Pi1基因和Pi2基因双基因纯合且农艺性状优良的水稻新品系，在湖北省水稻品种区域试验的稻瘟病抗性鉴定点恩施和宜昌分别对这些品系的稻瘟病抗性进行了田间鉴定。[结果]这些品系的抗性综合指数在0.8～2.0，抗级为1，表现为抗，而受体亲本扬稻6号的抗性综合指数分别为8.8和8.5，抗级为9，表现为高感，其中一个品系R587与不育系广占63S配制的杂交组合的抗性综合指数分别为2.5和3.5，抗级为3，表现为中抗，而对照扬两优6号的抗性综合指数分别为7.9和8.2，抗级为9，表现为高感。[结论]通过分子标记辅助选择聚合Pi1基因和Pi2基因能显著提高聚合系及其相应杂交组合的稻瘟病抗性。

关键词 水稻；稻瘟病；分子标记辅助选择；抗性鉴定

中图分类号 S511 文献标识码

A 文章编号 0517-6611（2015）05-012-03

Development and Evaluation of Disease Resistance of Pyramided Lines with Blast Resistance Genes Pi1 and Pi2 in Rice

LI Jinbo，XIA Mingyuan，QI Huaxiong （Institute of Food Crops， Hubei Academy of Agricultural Sciences， Wuhan， Hubei 430064）

Abstract [Objective] The aim was to develop and evaluate disease resistance of pyramided lines with blast resistance genes Pi1 and Pi2 in rice.[Method] Using GD7 as donors as rice blast resistance genes， and Yangdao 6 as recipient， ten new genepyramided lines with the rice blast resistance genes Pi1 and Pi2 and desirable agronomic traits were developed by bakecross breeding with molecular markerassisted selection （MAS）. The field resistance of these lines and their original parents was identified in Ensi and Yichang of Hubei. The evaluation of these lines was conducted on the resistance to blast. [Result]The results showed that the disease index of these lines were 0.8-2.0 and they were resistant. And the disease index of Yangdao 6 was 8.8 and 8.5 and it was high susceptible. The disease index of Guangzhan63S/R587 was 2.5 and 3.5 and it was moderately resistant. And the disease index of Yangliangyou 6 was 7.9 and 8.2 and it was high susceptible. [Conclusion]The conclusion was drawn that the field resistance of genepyramided lines and their hybrid was significantly increased by MAS.

Key words Rice； Blast； MAS； Evaluation of disease resistance

基金項目 湖北省农业科技创新中心资助项目（2011620002001）。

作者简介 李进波（1971- ），男，湖北天门人，副研究员，硕士，从事水稻分子育种研究。

收稿日期 20150114

由稻瘟病菌（Magnaporthe oryzae）引起的稻瘟病是一种世界性的稻作病害，在世界各个水稻生产国或地区每年都有不同程度的发生[1]。在我国每年都有几个稻作区中等或偏重流行，且过去20年有增长趋势[2-3]。据全国农作物重大病虫害预警，2012年稻瘟病在我国发病面积为533万hm2，较2011年增加20%[4]。实践证明，在众多的防治措施中抗病品种的培育和种植是控制该病害最为经济、安全和有效的方法[5]。……