宋娇　杨烨　喻小琼等

摘要

[目的]建立肌卫星细胞和肌内前体脂肪细胞的共培养体系。[方法]用10 μg/ml INS、1 μmol/L DEX和115 ng/ml IBMX对北京油鸡肌肉SV细胞进行诱导后，通过油红染色法、细胞免疫学鉴定及关键基因的表达对诱导的SV细胞进行分析。[结果]油红O脂肪染色法鉴定结果表明，诱导后的细胞红色脂质密度较高，说明SV细胞经过诱导后含有更多的前体脂肪细胞。通过实时荧光PCR定量检测细胞发育的标志性基因，发现诱导与未诱导的肌肉SV细胞均会表达前体脂肪细胞标志性基因。[结论]肌肉SV细胞经过诱导后前体脂肪细胞的含量得到提高，有利于建立前体脂肪细胞和肌卫星细胞的体外培养体系。

关键词 北京油鸡；前体脂肪细胞；肌卫星细胞；共培养体系；诱导

中图分类号 S831.8+9 文献标识码 A 文章编号 0517-6611（2015）15-151-03

Study on the Adipogenic Differentiation of SV Cells in Skeletal Muscles of Beijing You Chicken

SONG Jiao1， YANG Ye1*， YU Xiaoqiong2 et al

（1. College of Animal Science， Yangtze University， Jingzhou， Hubei 434025； 2. State Key Laboratory of Animal Nutrition， Institute of Animal Sciences of Chinese Academy of Agricultural Sciences， Beijing 100193）

Abstract [Objective] This research aimed to establish the cocultures system of muscle satellite cells and intramuscular preadipocytes. [Method] The SV cells in the muscles of Beijing You Chicken were induced with 10 μg/ml insulin （INS）， 1 μmol/L dexamethasone （DEX） and 115 ng/ml 3isobutyl1methyl xanthine （IBMX）. And the induced SV cells were identified by oil red O staining， cellular immunological identification and the expression of key genes involved in adipogenesis and myogenesis. [Result] The results of oil red O staining showed that the induced SV cells contained higherdensity red stained lipid， which indicated that the induced SV cells contained more preadipocytes. The key genes about the cell development were detected by realtime fluorescent quantitive PCR. The detection results showed that the key genes of preadipocytes could be expressed by induced and noninduced SV cells in the muscles. [Conclusion] The induced muscle SV cells contained more preadipocytes， which was be propitious to establish the coculture system in vitro of muscle satellite cells and preadipocytes.

Key words Beijing You Chicken； Preadipocyte； Muscle satellite cell； Coculture system； Induction

肌內前体脂肪是肌卫星细胞的重要组成部分之一，肌卫星细胞在特定的分子机制（如PPARs信号系统、WNT信号系统和肌肉分泌因子）作用下可以转变为脂肪细胞[1]。由于受到肌肉和脂肪内分泌素（myokines、adipokines）相互作用的影响，肌内脂肪细胞可能与脂肪组织里脂肪细胞具有不同的生物学代谢过程[2]。在共培养体系中，肌细胞和脂肪细胞的代谢都与单独培养时的代谢具有一定的差异[3]。因此，建立合适的共培养体系能更好地阐明细胞间相互联系的复杂性，是研究肌内脂肪沉积和肌肉发育的生物学基础。

来源于肌肉的SV细胞具有类似于肌卫星细胞的增殖分化特性，同时也具有一些脂肪细胞……