陈关雄　姜学云　台晓媛等

摘要 [目的] 了解云南省副猪嗜血杆菌流行毒株的生化特性。[方法] 采集10頭6月龄疑似猪传染性副猪嗜血杆菌病的病猪样品， 用巧克力培养基进行细菌的分离与培养，并对分离菌株进行细菌的形态观察、培养特性和生化试验鉴定。[结果] 成功分离出1株副猪嗜血杆菌，该分离株不需要X因子，但需要V因子。分离菌株被鉴定为副猪嗜血杆菌，从而将此病例确诊为副猪嗜血杆菌病。[结论] 该研究可为进一步控制副猪嗜血杆菌的危害奠定基础。

关键词 副猪嗜血杆菌；分离；鉴定；生化特性

中图分类号 S858.28 文献标识码 A 文章编号 0517-6611（2015）15-159-02

Isolation and Identification of Epidemic Strains of Swine Heamophilus parasuis in Yunnan Province

CHEN Guanxiong1，JIANG Xueyun2，TAI Xiaoyuan3，ZHU Lingyun5* et al

（1. Animal Husbandry and Veterinary Station in Shilin County of Yunnan Province， Shilin， Yunan 652200； 2. Comprehensive Agricultural Service Center of Xijiekou Town in Shilin County of Yunnan Province， Shilin， Yunan 652209； 3. Comprehensive Agricultural Service Center in Dake Township of Shilin County，Yunnan Province，Shilin，Yunan 652202；5. College of Animal Science and Technology， Yunnan Agricultural University， Kunming， Yunan 650201）

Abstract [Objective] The research aimed to understand the biochemical characteristics of epidemic strains of swine Heamophilus parasuis in Yunnan Province. [Method] The diseased samples were collected 10 heads of suspected swine infected with H. parasuis. The pathogen was isolated and cultured with chocolate medium. And the isolated strain was identified by using morphologic observation， culture characteristics and biochemical tests. [Result] One strain of H. parasuis was successfully isolated. This isolated strain didnt need X factor， but it needed V factor. [Conclusion] The research laid the foundation for controlling the damage of H. parasuis further.

Key words Heamophilus parasuis；Isolation； Identification；Biochemical characteristics

副猪嗜血杆菌病（Haemophilus parasuis，Hps）又称为猪革拉斯氏病（Glassers disease），常引起猪的多发性浆膜炎和关节炎，曾被认为是由应激所引起的散发性疾病，后来被证实是由副猪嗜血杆菌所引起[1]。副猪嗜血杆菌可以影响从2周龄到4月龄的青年猪，主要在断奶后和保育阶段发病，通常见于5～8周龄的猪，发病率一般为10%～15%，严重时死亡率可达50%[2]。

随着世界养猪业的发展，该病已成为全球范围内影响养猪业的典型细菌性疾病[3]。在我国，由于饲养技术的调整不当以及突发新的呼吸道综合征，使得该病日趋流行，危害日渐严重。近年来，我国各猪场发生多发性浆膜炎和关节炎的报道屡见不鲜，其中不少病例的病原被初步诊断为副猪嗜血杆菌，但限于生产条件，极少有人对其进行确切诊断，因此有必要对其进行实验室研究，确定其生物特性。

笔者从具……