淮山水提物抗氧化活性的研究
2015-07-13周畅周浓刘亚等
周畅 周浓 刘亚等
摘要 [目的]采用淮山为原料,研究淮山水提物的抗氧化活性。[方法]以淮山水提液对超氧阴离子、羟基自由基的清除率为考察指标,通过单因素和正交试验优化水提工艺。[结果]最佳水提工艺条件为提取温度70 ℃、液料比60∶1、提取时间160 min,在此工艺条件下,淮山水提物对超氧阴离子的清除率为11.15%,对羟基自由基的清除率为13.06%。[结论]淮山水提物对羟基和超氧阴离子的自由基均表现出较强的清除能力,具有较强的抗氧化活性。
关键词 淮山;水提液;抗氧化活性;优化工艺
中图分类号 S53 文献标识码 A 文章编号 0517-6611(2015)15-069-03
Study on the Antioxidant Activity of Aqueous Extract from Dioscorea fordii Prain et Burkill
ZHOU Chang,ZHOU Nong,LIU Ya* et al
(Agricultural College, Guangdong Ocean University, Zhanjiang, GuangDong 524088)
Abstract [Objective]Taking Dioscorea fordii Prain et Burkill as material, the antioxidant activities of its aqueous extracts were studied. [Method]Using the scavenging rate of superoxide anion and hydroxyl radical as indices,the single factor and orthogonal experimental design were employed to optimize the extracting technology for Dioscorea fordii Prain et Burkill by hot water.[Result]The optimum conditions was 70 ℃, an liquid-to-solid ratio of 60∶1, 160 min. The scavenging rate of superoxide anion and hydroxyl radical of aqueous extract were 1115% and 13.06% respectively. [Conclusion]The aqueous extract of Dioscorea fordii Prain et Burkill had great scavenging ability to superoxide anion and hydroxyl radical, indicating strong antioxidant activities.
Key words Dioscorea fordii Prain et Burkill;Aqueous extract;Antioxidant activity;Optimization of the technology
淮山又名山药、薯蓣,为薯蓣科植物,其分布的区域极广,遍及热带、亚热带等地区。由于栽种方式或因地区差异、品种差异,物种也是十分繁杂。淮山每年的产量均非常大。主要以其根块来提供食用,是我国传统的药食两用食物之一,也在我国保健食品中占有重要地位。淮山的根块肉质肥厚,且营养价值很高,研究表明,每100.00 g鲜山药中含蛋白质3.37 g、淀粉15.58 g、灰分0.70 g[1]。《神农本草经》[2]概括了淮山的五大功用“益肾气,健脾胃,止泄痢,化痰涎,润皮毛”,淮山可治疗脾胃虚弱、泄泻、体倦、食少、虚汗等病症。淮山皂苷成分具有明显的药理作用,如改善心脑血液循环 、防止心律失常、双向调节免疫力、抗缺氧和抗疲劳、抑制神经递质释放和调节腺苷酸环化酶活性等[3-4]。尿囊素是淮山的活性成分之一,它具有抗刺激、麻醉镇痛、消炎抑菌等作用,常用于治疗手足皴裂、鱼鳞病以及多种角化性皮肤病。……
