广西金秀香芋无菌芽增殖的相关因子研究
2015-07-13韦鹏霄蒋含笑岑秀芬
韦鹏霄 蒋含笑 岑秀芬
摘要 [目的]探讨影响广西金秀香芋无菌芽增殖的相关因子。[方法]以广西金秀香芋无菌芽为材料,从不同基本培养基、不同生长素种类、不同6BA浓度、不同激素组合及不同蔗糖浓度等对广西金秀香芋无菌芽继代增殖进行比较。[结果]无菌芽继代增殖的适宜基本培养基为MS培养基,适宜的生长素为0.2 mg/L NAA,适宜的6BA浓度为4.0 mg/L,适宜的激素组合为0.2 mg/NAA+3.0 mg/L 6BA+0.5 mg/L PP333,最佳的蔗糖浓度为30 g/L。[结论]不同基本培养基、不同生长素种类、不同6BA浓度、不同激素组合及不同蔗糖浓度对广西金秀香芋无菌芽增殖的影响不同。
关键词 金秀香芋;无菌芽;芽增殖;相关因子
中图分类号 S504.3 文献标识码 A 文章编号 0517-6611(2015)15-032-03
Study on Correlation Factors for Sterile Buds Multiplication of Jinxiu Taro in Guangxi
WEI Pengxiao, JIANG Hanxiao, CEN Xiufen
(Agricultural College, Guangxi University, Nanning, Guangxi 530005)
Abstract [Objective] The purpose of this experiment is to investigate the correlation factors affecting the sterile buds multiplication of Jinxiu taro in Guangxi. [Method] The sterile buds of Jinxiu taro was used as the materials and the comparative experiment was carried on different basic mediums, auxin kinds, 6BA concentrations, hormones combinations and sugar concentrations. [Result] In the subculture multiplication of sterile buds, the suitable basic medium was MS medium, the auxin kind was 0.2 mg/L NAA, the 6BA concentration was 4.0 mg/L, the hormones combination was 0.2 mg/L NAA+0.3 mg/L 6BA +0.5 mg/L PP333 and the sugar concentration was 30 g/L. [Conclusion] The different basic mediums, auxin kinds, 6BA concentration, hormones combinations and sugar concentrations showed different effects on sterile buds multiplication of Jinxiu taro in Guangxi.
Key words Jinxiu taro; Sterile buds; Bud multiplication; Correlation factors
广西金秀香芋属天南星科芋属魁芋类型,是槟榔芋的一个优良品种,亦是金秀县农民种植的主要经济作物之一。其含有丰富的淀粉和多種维生素,既营养丰富,又香味浓郁,是饮食点心的上乘原料,球茎可食用也可入药;叶柄可作菜,也可作家畜饲料[1],因而深受人们喜爱。
然而,由于长期利用球茎进行营养繁殖,栽培芋已普遍遭受病毒浸染。徐炯志等[2]研究表明,长期以球茎作为资源繁殖,难免品种退化,产量、品质变劣。目前芋病毒中分布最广、危害最大的是芋花叶病毒,感染此种病毒的植株生活力降低,球茎大小、数量和品质均下降,产量损失接近60%[3-7]。因此,利用离体快繁技术,研究影响金秀香芋无菌芽增殖的因子,以及如何快速大量获得金秀香芋无菌苗,对降低金秀香芋的种植成本和提高其品质具有重要意义。……
