雨生红球藻中虾青素的提取工艺优化
2015-07-13李小慧邹宁孙东红等
李小慧 邹宁 孙东红等
摘要
[目的]探索大规模工业生产适用的虾青素提取方法。[方法]从原料的状态入手,采用常规的有机溶剂——丙酮作提取剂,对新鲜雨生红球藻膏和干藻粉中虾青素的提取率进行比较。[结果]使用目前采用的提取条件,从干藻粉中得到的虾青素提取率为新鲜藻膏的2倍多。[结论]烘干后的雨生红球藻在虾青素的提取中具有更大的优势。
关键词 雨生红球藻;虾青素;新鲜藻膏;干藻粉;提取工艺优化
中图分类号 S188 文献标识码
A 文章编号 0517-6611(2015)15-023-02
Optimization of Productional Astaxanthin Extraction Process from Haematococcus pluvialis
LI Xiaohui, ZOU Ning,SUN Donghong et al (Lu Dong Univercity of Life Science,Yantai,Shandong 264025)
Abstract [Objective]In order to explore the applicative extraction process of largescale production of astaxanthi. [Method] The different states of Haematococcus material were compared in this work. With conventional organic solvent as extracting agent, the extraction rates of astaxanthin from fresh paste and dry power of Haematococcus pluvialis were compared. [Result]The results showed that,at the current extraction conditions, the extraction rate of astaxanthin obtained from dry powder was about 2 times more than fresh paste. [Conclusion] Dry Haematococcus pluvialis had more advantages in Productional astaxanthin extraction process.
Key words Haematococcus pluvialis; Astaxanthin; Fresh paste; Dried powder; Optimization of extraction process
雨生红球藻中虾青素的含量高达1.5%~3.0%。虾青素是一种高效纯天然抗氧化剂,其主要作用是清除人体内的自由基,并能提高人体抗衰老能力。目前,人们公认的天然虾青素的生物来源有3种:虾、蟹等水产品的废弃物,红发夫酵母及雨生红球藻。其中,虾、蟹等水产品的废弃物中,虾青素不仅含量低,而且提取费用高;而天然红发夫酵母中虾青素的平均含量仅为0.4%;因此,雨生红球藻被确认为自然界中生产天然虾青素的最好生物来源[1]。其天然抗氧化性是类胡萝卜素的10倍、VE的550倍。因此,虾青素在保健食品、药品、高档化妆品、生物医药制剂以及饲料添加剂等领域有很大需求和应用前景。
近年來,随着对雨生红球藻研究的深入开发和发展,越发凸显出其经济和利用价值,其社会地位和开发价值也日趋上升。而目前最关键的是对虾青素的生产性提取工艺进行优化。大规模生产虾青素的提取费用很高,从而制约了利用雨生红球藻生产虾青素的开发和利用,因此,虾青素的提取工艺优化迫在眉睫。……
