杨磊　艾鑫正　黄文邺等

摘要

[目的]分析恩施有代表性的4种农作物不同部位微量元素含量。[方法]采用HNO3H2O2体系高压密闭消解4种农作物不同部位，电感耦合等离子体质谱联合碰撞反应池法， 测定其中微量元素，以铑和铼内标法补偿校正，以国家标准物质验证方法的准确性。[结果] 该方法基体效应弱，线性相关系数均不小于0.999 8，方法的检出限范围是0.014～0.053 μg/L，精密度（RSD）在0.53%～1.52%之间，加标回收率在98.65%～101.37%之间。[结论] 该方法检测高效、准确，适合农作物中多种元素的同时测定，同时研究了4种农作物中微量元素的分布，发现不同农作物微量元素含量不同，而同一农作物不同部位中微量元素含量也有一定差异。该研究首次分析了恩施富硒地区4种农作物中硒与5种微量元素的相关性，为研究富硒农产品中富硒程度的变化与其他微量元素是否处于安全且营养的范围提供科学依据。

关键词 HNO3H2O2；高压密闭消解；电感耦合等离子体质谱；农作物；微量元素

中图分类号 S126 文献标识码 A 文章编号 0517-6611（2015）15-005-03

Determination of Trace Elements in Four Kinds of Typical Crops in Enshi by ICPMS with High Pressure Sealed Digestion and Study of Their Relationship

YANG Lei， AI Xinzheng， HUANG Wenye， LIAO ZhaoJiang*

（College of Biology and Pharmacy， China Three Gorges University， Yichang， Hubei 443002）

Abstract [Objective] The research aimed to determine the contents of trace elements in four kinds of typical crops in Enshi. [Method] Inductively coupled plasma mass spectrometry （ICPMS） with high pressure sealed digestion was adopted. The samples were digested by a mixed reagent of HNO3H2O2 sealed vessels. Rh and Re were selected as internal standard to compensate the analytical signal drift. The method was verified by national standard substance elements. [Result] Matrix effect of the method was weak and the linear correlation coefficient wasnt less than 0.999 8. The limit of detection range was 0.014-0.053 μg/L， RSD was 0.53%-1.52%， and standard addition recovery rate was 9865%-101.37%. [Conclusion] The method was effective and accurate， which was suitable for the detection of the trace elements in crops. It was found that trace elements content in different crops were different， and trace elements content in different parts of the same crops had certain differences. The relationship of five trace elements and Se in four kinds of typical crops in Enshi was analyzed for the first time， and the scientific basis in safe and nutrition range was provided for the research scope of the change of Se in agricultural products and other trace elements.

Key words HNO3H2O2； High pressure sealed digestion； ICPMS； Crops； Trace elements

聯合国粮农组织/世界卫生组织（FAO/WHO）将人体微量元素分为三类。第一类为人体必需微量元素，其中包括硒（Se）、锌（Zn）、铜（Cu）、钼（Mo）、铬（Cr）、钴（Co）。它们在人体生理活动中有着不可替代的调控作用，与人的健康、寿命、疾病、智力等密切相关[1-4]，如硒与抗氧化，锌与生长发育，铜与造血，钼与酶活性，铬与糖尿病，钴与红细胞生成等。而人体主要通过食物补充来获得这些微量元素，尤其是硒元素。

微量元素常见的检测方法有电感耦合等离子体发射光谱法（ICPAES）[5-6]、原子吸收光谱法（AAS）[7-8]、电感耦合等离子体质谱法（ICPMS）[9-10]和一些联用技术等[10-11]。……