石志红 张慧峰

[摘要] 目的：通过测定慢性阻塞性肺疾病（COPD）患者肺组织中水通道蛋白5（AQP）、钠泵α1亚单位蛋白的表达及与肺功能变化的关系，明确AQP5、钠泵α1亚单位在COPD发生发展中的作用。方法：选取肺叶切除术患者肺组织标本52例，其中，对照组25例，COPD组27例；免疫组化法检测AQP5、钠泵α1亚单位蛋白表达。结果：与对照组相比，AQP5蛋白在COPD组表达降低（P＜0.05），在COPD中度组较轻度组表达亦降低，两组比较，差异有统计学意义（P＜0.05）。AQP5表达改变与FEV1/FVC、FEV1预计值呈正相关关系（Rs=0.717，P＜0.05；Rs=0.500，P＜0.01）。钠泵α1亚单位在COPD组患者的表达与对照组相比差异无统计学意义（P>0.05）。结论：AQP5参与了COPD患者气道黏液分泌的病理过程并与COPD气流受限有关，钠泵α1亚单位在COPD的发病机制中可能起辅助作用。

[关键词] 慢性阻塞性肺疾病；水通道蛋白5；钠泵；黏液纤毛功能障碍

[中图分类号] R563[文献标识码]A [文章编号]1673-7210（2011）11（c）-031-03

Changes and clinical significance of the expression of Aquaporin 5 and sodium pump α1 subunit in the airway of patients with chronic obstructive pulmonary disease

SHI Zhihong1, ZHANG Huifeng2

1.Department of Respiration Medicine, the First Affiliated Hospital of Medical College in Xi′an Jiaotong University, Shaanxi Province, Xi′an 710061, China; 2.Department of Intensive Medicine (ICU), He′nan Provincal People′s Hospital, Zhengzhou 450003, China

[Abstract] Objective: To study the role of AQP5 and sodium pump α1 subunit in development of COPD by measuring AQP5 and sodium pump α1 subunit protein expression and their relationships with pulmonary function changes in COPD patients. Methods: The sample from 52 cases of pulmonary lobectomy patients, including 25 cases of control group and 27 cases of COPD group, each sample was stained by immunohistochemical staining to detect AQP5 and sodium pump α1 subunit protein expression. Results: Compared with the control group, AQP5 protein expression decreased in the COPD group (P<0.05); moreover, compared with mild group, AQP5 also decreased in moderate COPD group (P<0.05). Change of AQP5 expression was positively correlated with predicted values of FEV1/FVC and FEV1 (Rs=0.717, P<0.05; Rs=0.500, P<0.01); sodium pump α1 subunit expression in COPD patients showed no significant difference compared with the control group (P>0.05). Conclusion: AQP5 participates in pathological process of airway mucus secretion in COPD patients and has the relationgship with airway obstruction of COPD; sodium pump α1 subunit may play a minor role in the pathogenesis of COPD.

[Key words] Chronic obstructive pulmonary disease; Aquaporin 5; Sodium pump; Mucociliary dysfunction

慢性阻塞性肺疾病（chronic obstructive pulmonary diseases，COPD）是常见的慢性呼吸系统疾病，COPD的气道炎症、结构改变和黏液纤毛功能障碍等可引起气流受限导致肺功能下降，临床症状加重和健康状态恶化[1]。气道黏液纤毛清除功能障碍是COPD的一个重要病理生理改变，促进了COPD的发生、发展。水通道蛋白5（Aquaporin 5，AQP5）、钠泵是气道上皮主要的离子转运通道，与气道表面液体（aairway surface liquid，ASL）的分泌和调节有密切关系，本研究以COPD患者手术标本为研究对象，探讨肺组织中AQP5、钠泵α1亚单位蛋白的表达及与肺功能变化的关系，明确两者在COPD发生发展中的作用机制。

1 材料与方法

1.1 材料

肺组织标本来源于我院胸外科和肿瘤外科2005年4月～2006年4月因肺部肿瘤行肺叶切除术的患者。所……