桑葚汁花青素提取工艺优化

2025-08-07赵书田熊灵丁波林颖吴浪张文学

中国调味品 2025年7期

DOI：10.3969/j.issn.1000-9973.2025.07.031

中图分类号：TS255.36 文献标志码：A 文章编号：1000-9973（2025）07-0210-08

Optimization of Extraction Process of Anthocyanins from Mulberry Juice ZHAO Shu-tian¹，XIONG Ling1，DING Bo1，LIN Ying1，WU Lang²， ZHANG Wen-xue1，3 * （1.College of Biomass Science and Engineering， Sichuan University，Chengdu 6loo65，China; 2.Panzhihua Sixi Agricultural Development Co.，Ltd.，Panzhihua 6l7ooo，China; 3.School of Liquor-Brewing Engineering，Sichuan University Jinjiang College，Meishan 62086o，China）

Abstract： In order to obtain mulberry anthocyanins，response surface method is used to optimize the processing technology of mulberry juice， and then the macroporous adsorption resin is used to adsorb anthocyanins in mulberry juice，so as to explore the effects of diffrent types of macroporous resins and different process parameters on the extraction of anthocyanins from mulberry juice. The results show that after response surface method optimization， the juice yield of mulberry juice is （75.01±0.37）% HPD60O macroporous resin is more suitable for extracting anthocyanins from mulberry juice. The best process parameters are static adsorption time of 6h ， the most suitable ethanol solution pH value of 3，concentration of 70% ; dynamic adsorption flow rate of 3mL/min ，which can continuously adsorb 22BV mulberry juice；using 8 BV desorbed solution can basically desorb the anthocyanins on the macroporous resin. Under the above conditions，the extraction rate of anthocyanins from mulberry juice is （49.30±1.23）% .It is feasible to use macroporous resin to adsorb anthocyanins from mulberry juice，which has provided a theoretical basis for the industrial use of anthocyanins.

eywords：mulberry；macroporous resin；anthocyanins；extraction

桑葚（Fructusmorispp.）是桑树的成熟果穗[1]中国的桑葚品种丰富，尤其以云、贵、川三省的种类最丰富，产量多[2]。成熟桑葚中含有丰富的花青素，营养价值较高[3]，对人体生理机能具有调节作用，如保护视力、抑制癌细胞增殖[4]、抗氧化[5]等。目前，大多数研究都使用溶剂法提取果干或新鲜桑葚中的花青素，该方法溶剂用量大、成本较高，且提取的花青素需进一步纯化。大孔树脂是一种新型有机高聚物吸附剂，根据其极性可分为极性、非极性、弱极性3种，对有机化合物具有较高的吸附能力，具有吸附量大、低成本、可再生等优点[8-9]。目前，使用大孔树脂对果汁中色素的提取研究很多，适宜工厂大规模生产。

本研究首先对桑葚汁的出汁率进行优化，再使用不同型号的大孔树脂从桑葚汁中回收部分花青素，得到桑葚汁花青素提取最适大孔树脂型号和最适工艺参数，为桑葚花青素提取的工业化发展提供了理论基础。

1材料与方法

1.1 主要材料与试剂

新鲜桑葚（云桑2号）：产自省凉山州；大孔树脂（HPD600、D101、AB-8）：上海源叶生物科技有限公司；无水乙醇、盐酸、氢氧化钠、乙酸钠、氯化钾（均为分析纯）：成都金山化学试剂有限公司；果胶酶、纤维素酶、木聚糖酶：河南省万邦实业有限公司。