迟庆雷 王利存 汪丰海 于琪

摘要 [目的]为提高微藻光生物反应器的培养参数控制能力，建立起先进、稳定的微藻培养测控系统，进而提高微藻生产产量和品质。[方法]通过分析微藻培养过程参数确定拟设计综合测控系统的监测参数和控制参数，设计并研制出通用型综合测控系统，开展完整批次的微藻培养测试。[结果]测控系统稳定运行，光照、温度、pH、溶解氧的变化趋势相互印证，叶绿素荧光参数和RGB谱很好地反映出微藻细胞光合系统的生理状态，系统测量的自动OD与干重都有很好的线性关系，可以直接反映微藻生长密度。[结论]微藻综合测控系统设计合理、运行稳定、自动化程度高，很好地反映了微藻生产过程中的状态和趋势，实现了微藻生产过程的可控培养。

关键词 微藻培养;光生物反应器;测控系统;培养试验

中图分类号 TP 273  文献标识码 A  文章编号 0517-6611（2021）21-0218-05

doi：10.3969/j.issn.0517-6611.2021.21.056

开放科学（资源服务）标识码（OSID）：

Development and Test of Integrated Measurement and Control System for Microalgae Cultivation Process

CHI Qing-lei  WANG Li-cun  WANG Feng-hai 2 et al

（1. SDIC Biotech Investment Co.， Ltd.， Beijing 100032;2.Beijing Key Laboratory of Microalgae Bioenergy and Resources， Beijing 100032）

Abstract [Objective]In order to improve the culture parameter control ability of the microalgae photobioreactor， an advanced and stable microalgae culture measurement and control system has been established to improve the microalgae production yield and quality.[Method] Through analyzing the parameters of the microalgae cultivation process， the monitoring parameters and control parameters of the proposed integrated measurement and control system were defined， and a universal integrated measurement and control system was designed and developed. Then a complete microalgae cultivation test was carried out. [Result]The system was running stably， and the changing trends of light， temperature， pH and dissolved oxygen were mutually confirmed. The chlorophyll fluorescence parameters and RGB spectrum well reflected the physiological state of the microalgae cell photosynthesis system. The automatic OD measured by the system had a good linear relationship with the dry weight， which could directly reflect the growth density of microalgae. [Conclusion]The microalgae integrated measurement and control system has reasonable design， stable operation and high degree of automation， which well reflects the status and trend of the microalgae production process， and realizes the controllable cultivation of the microalgae production process.

Key words Microalgae culture;Photobioreactor;Measurement and control system;Culture experiment

基金項目 北京市科技计划项目（Z141100000614011）;国家开发投资公司资助项目“高效经济光生物反应器研发”。

作者简介 迟庆雷（1981—），男，山东日照人，高级工程师，硕士，从事微藻全流程工艺与装备研究。

收稿日期 2021-06-06

微藻作为光合自养微生物，由于其光合作用效率高、细胞生长快、不占用耕地、可连续生产等优点，被认为是营养品、食品、医药、生物基化学品以及可持续生物质能源生产的原料来源[1-3]。微藻产业化应用的前提是获得低成本的微藻生物质原料，而其中的核心是精准可控的微藻大规模培养。……