王兴珍 卯旭辉 贾秀苹 梁根生

摘要 以55份油葵种质资源为材料，采用浓度为20%的聚乙二醇（PEG-6000）模拟干旱胁迫，以清水为对照，研究干旱胁迫下不同油葵种质资源萌发期抗旱特性的差异。结果表明，20% PEG-6000 胁迫下，不同资源的相对发芽势、相对发芽率、相对胚芽长、相对胚根长、相对胚根数、相对鲜重6个指标表现并不一致，抗旱性评价需要进行多指标的综合考虑。采用隶属函数值和抗旱指标权重相结合的抗旱性度量值进行抗旱性评价，55个品种的D值在0.368～0.730，差异明显。并采用系统聚类分析的组间连接（平方欧式距离）法对品种综合能力D值进行聚类分析，将55份材料分为 3 类：TK3303、PR2301和FK01为第Ⅰ类（属强抗旱类型），占供试材料的 5.45%;九洋918为第Ⅱ类（属中等类型）;其余品种为第Ⅲ类（属弱抗旱类型）。TK3303、PR2301和FK01可作为今后油葵抗旱育种的优异种质资源。

关键词 油葵;种质资源;种子萌发;抗旱性

中图分类号 S 565.5  文献标识码 A  文章编号 0517-6611（2021）20-0040-05

doi：10.3969/j.issn.0517-6611.2021.20.011

开放科学（资源服务）标识码（OSID）：

Evaluation of Drought Resistance of Oil Sunflower Germplasm Resources at Germination Stage under Polyethylene Glycol Simulated Drought Stress

WANG Xing-zhen， MAO Xu-hui， JIA Xiu-ping  et al

（Crop Research Institute， Gansu Academy of Agricultural Sciences， Lanzhou， Gansu 730070）

Abstract 55 oil sunflower germplasms were used as materials to study the difference of drought resistance characteristics of different oil sunflower germplasms at germination stage under drought stress by simulating drought stress with 20% polyethylene glycol （PEG-6000） and water as control. The results indicated that under 20% PEG-6000 stress， the relative germination potential， relative germination rate， relative germ length， relative radicle length， relative radicle number and relative fresh weight of different resources were inconsistent， and drought resistance evaluation needed comprehensive consideration of multiple indicators.The drought resistance measurement value combining the membership function value and the drought resistance index weight was used to evaluate the drought resistance. The D values of 55 varieties were between 0.306 and 0.730， and the difference was obvious.The inter-group connection （squared euclidean distance） method of hierarchical cluster analysis was used to cluster the D value of comprehensive ability of cultivars. The 55 cultivars were divided into 3 categories： TK3303， PR2301 and FK01 were the first category （strong drought resistance type）， which accounted for 5.45% of the tested materials;Jiuyang 918 was classified as type II （medium type）;the rest were classified as type III （weak drought resistance type）.TK3303， PR2301 and FK01 could be used as excellent germplasm resources for drought resistance breeding of oil sunflower in the future.

Key words Oil sunflower;Germplasm resources;Seed germination;Drought resistance

基金项目 国家现代农业产业技术体系资助（CARS-14-2-22）;甘肃省青年科技基金計划（21JR1RA357）。

作者简介 王兴珍（1987—），女，甘肃白银人，助理研究员，硕士，从事向日葵遗传育种研究。*通信作者，副研究员，从事高产向日葵育种研究。

收稿日期 2021-03-02

随着全球气候变暖，干旱已成为世界农业生产长期面临的主要制约因素，据估计，因干旱造成的农业损失相当于其他不良因子造成的损失总和[1]。目前，全世界遭受水土流失的耕地面积约2.5×109 hm2[2]，严重影响了农作物的生长，制约了农业经济的发展[3]。……