卢少芬 杜明仪 谭晓颖 龙海燕 陈雪英 张琦 陈馥如 盘琳

[摘要]目的探討全血过滤白细胞时间长短对血液质量的影响。方法选取2020年4月至2021年3月广东省肇庆市中心血站使用上海输血技术有限公司生产的一次性使用去白细胞滤器血袋 T-200采集的200袋全血，按照其血袋说明书要求进行当天过滤操作，并对其过滤时间实施监控，分别按滤除时间为5 min、10 min、15 min、20 min 划分成四组，收集400份滤前及滤后血液样本作为研究对象，通过比较过滤前后的全血容量、白细胞计数、血红蛋白含量及储存期末溶血率等指标差异，分析全血过滤白细胞时间长短对血液质量的影响。结果①随着全血过滤白细胞时间的增加，过滤后血液容量无显著变化，差异无统计学意义（ P >0.05），但血液当中白细胞数量明显减少，差异有统计学意义（ P <0.05）;②随着全血过滤白细胞时间的增加，过滤后血液当中的血红蛋白含量无明显变化，差异无统计学意义（ P >0.05），但储存期末溶血率逐渐升高，差异有统计学意义（ P <0.05），过滤时间20 min 有出现储存期末溶血率>0.8%、质量检测不达标的情况。结论全血过滤时间长短对血液质量有明显的影响，为了保证去白细胞血液的制备质量，

200 ml 全血过滤白细胞时间最佳在10min，最长不宜超过20 min。[关键词]全血过滤;白细胞;容量;白细胞计数;血红蛋白;溶血率

[中图分类号] R457  [文献标识码] A   [文章编号]2095-0616（2021）23-0186-04

Study on the effect of filtration time of leukocyte in whole blood on blood quality

LU  Shaofen    DU  Mingyi    TAN  Xiaoying    LONG  Haiyan    CHEN  Xueying    ZHANG  QiCHEN  Furu    PAN  Lin

Central Blood Station of Zhaoqing City in Guangdong Province， Guangdong， Zhaoqing 526040， China

[Abstract] Objective To investigate the effect of filtration time of leukocyte in whole blood on blood quality. Methods A total of 200 bags of whole blood collected at the Central Blood Station of Zhaoqing City in Guangdong Province from April 2020 to March 2021， using the disposable leukocyte depletion filter blood bag T-200 produced by Shanghai Blood Transfusion Technology Co.， Ltd.， were filtered in the same day according to the instructions of the blood bag， and the filtration time was monitored. These collected bags were divided into 4 groups according to the filtration time of 5 min， 10 min， 15 min and 20 min. Collectively， 400 pre-filtration and post-filtration blood samples were collected as the objects. The differences of whole blood volume， leukocyte count， hemoglobin content and hemolysis rate at the end of storage were compared before and after filtration， and the effect of the filtration time of leukocyte in whole blood on blood quality was analyzed. Results ① With the increase of filtration time of leukocyte in whole blood， the blood volume did not change significantly after filtration， with no statistically significant difference （P >0.05）， but the leukocyte count in blood decreased significantly， with statistically significant difference （P <0.05）.② With the increase of filtration time of leukocyte in whole blood， the hemoglobin content in the whole blood had no significant change， with no statistically significant difference （P >0.05）， but the hemolysis rate at the end of the storage period gradually increased， with statistically significant difference （P <0.05）. After filtering for 20 min， the hemolysis rate at the end of storage period was more than 0.8%， and the quality did not meet the standard. Conclusion The length of whole blood filtration time has a significant effect on blood quality. In order to ensure the preparation quality of leukocyte-free blood， the best time to filter leukocytes from 200 ml of whole blood is 10 min and should not exceed 20 min.

[Key words] Whole blood filtration;Leukocyte; Volume;Leukocyte count;Hemoglobin;Hemolysis rate

过滤白细胞是输血界公认对临床输血有益的一项技术，国内许多血站及血液中心都开展了血液滤白的新技术和研究。目前去除血液中白细胞的方法有很多，其中以过滤法最为理想。过滤法指采用白细胞专用滤器滤除血液制剂中的白细胞，但滤除效果与所使用的滤器类型、血液制备方法、白细胞过滤时间等息息相关[1-2]。如何……