范冉 王文静 马捷 房忠军 闫旭

[摘要]目的比较 SYSMEX UF-1000i 与 SYSMEX UF-5000两种尿沉渣分析仪在白细胞（WBC）、红细胞（RBC）、管型（CAST）检测上的灵敏度、特异性以及与显微镜检查的一致性。方法随机收集2021年2—5月首都医科大学附属北京佑安医院病房及门诊患者1048份尿液标本。分别使用 SYSMEX UF-1000i、SYSMEX UF-5000以及显微镜进行检测，超过参考范围为阳性，否则为阴性，记录结果并进行比较分析。结果 SYSMEX UF-1000i 检测、SYSMEX UF-5000检测、镜检尿液 WBC 陽性率分别为20.04%、19.47%、17.56%; RBC 阳性率分别为17.37%、14.98%、11.83%; CAST 阳性率分别为35.31%、7.44%、6.77%。 SYSMEX UF-1000i、SYSMEX UF-5000检测相比较， WBC、RBC 结果差异无统计学意义（ P >0.05），CAST 结果差异有统计学意义（ P <0.05）。与镜检相比较，SYSMEX UF-1000i 检测 WBC 结果差异无统计学意义（ P >0.05），RBC、CAST 结果差异有统计学意义（ P <0.05）;SYSMEX UF-5000检测 WBC、CAST 结果差异无统计学意义（P >0.05），RBC 结果差异有统计学意义（P <0.05）。SYSMEX UF-5000较 SYSMEX UF-1000i 检测结果与镜检结果有更好的一致性。结论 SYSMEX UF-5000检测性能优于 SYSMEX UF-1000i，尿沉渣分析仪检测结果仍受多种因素影响，需结合显微镜检查提高检测准确性。

[关键词]尿沉渣分析仪;显微镜镜检;白细胞;红细胞;管型

[中图分类号] R446.12  [文献标识码] A   [文章编号]2095-0616（2021）23-0142-04

Comparison and analysis of the examinations with different urine sediment analyzers and microscopes

FAN  Ran    WANG  Wenjing    MA  Jie    FANG  Zhongjun    YAN  Xu

Clinical Laboratory Center， Beijing You'an Hospital Affiliated to Capital Medical University， Beijing 100069， China

[Abstract] Objective To compare the sensitivity and specificity of SYSMEX UF-1000i and SYSMEX UF-5000 urine sediment analyzers in the detection of white blood cell （WBC）， red blood cell （RBC） and cast （CAST）， and their consistency with microscopy. Methods A total of 1048 urine samples were collected randomly from the patients in wards and outpatient of Beijing You'an Hospital Affiliated to Capital Medical University from February to May 2021. SYSMEX UF-1000i， SYSMEX UF-5000 and microscopic examination were used for detection. If the value exceeds the reference range， it will be positive， otherwise it will be negative， and the results will be compared and analyzed. Results The positive rate of urine WBC in SYSMEX UF-1000i， SYSMEX UF-5000 and microscopic examination were 20.04%， 19.47%， 17.56%， respectively， the positive rate of RBC was 17.37%， 14.98%， 11.83%， respectively， and the positive rate of CAST was 35.31%， 7.44%， 6.77%， respectively. There were no significant differences in the results of WBC and RBC between SYSMEX UF-1000i and SYSMEX UF-5000（ P >0.05） ， but there was significant difference in the results of CAST （P <0.05）. Compared with microscopic examination， there was no significant difference in the results of WBC detected by SYSMEX UF-1000i （P >0.05）， but there were significant differences in the results of RBC and CAST （P <0.05）， and there were no significant differences in the results of WBC and CAST detected by SYSMEX UF-5000（ P >0.05） ， and there was significant difference in urine RBC （P <0.05）. Compared with SYSMEX UF-1000i， the test results of SYSMEX UF-5000 and the results of microscopy had better consistency. Conclusion SYSMEX UF-5000 has better detection performance than SYSMEX UF-1000i， and the results of urine sediment analyzer are still affected by many factors， so it is necessary to improve the detection accuracy by combining with microscopic examination.

[Key words] Urine sediment analyzer; Microscopic examination; White blood cell; Red blood cell; Cast

全自动尿沉渣分析仪可提高尿有形成分检测效率和标准化程度，近年有不少学者论证，尿沉渣分析仪对尿白细胞（WBC）、红细胞（RBC）的检测有较高的符合率，与显微镜检查（简称：镜检）相关性较好[1]。但管型（CAST）易受多重因素影響，仪器误检率较高[2]。本研究比较 SYSMEX UF-1000i 与 SYSMEX UF-5000仪器检测的有形成分结果并分析其干扰因素，旨在为检验工作者提供参考，以便更好地了解与应用尿沉渣分析仪。

1资料与方法

1.1一般资料

随机收集2021年2—5月首都医科大学附属北京佑安医院病房及门诊患者1048份尿液标本，其中男540例，女508例。已按相关流程向医院伦理委员会申请知情同意豁免。

1.2仪器与试剂

日本 SYSMEX UF-1000i 全自动尿沉渣分析仪及配套试剂：UF Ⅱ SEARCH-SED，批号：A0021;UF Ⅱ PACK-SED，批号：A0017; UF Ⅱ SEARCH- BAC，批号：A0028; UF Ⅱ PACK-BAC，批号： A0023; UF Ⅱ SHEATH，批号： G0054; SYSMEX 提供原装高、低值水平质控液批号：QC-YS0029。日本 ……