金美英 潘韦韦 崔镇海

[摘要]目的運用网络药理学探讨白芍治疗2型糖尿病的具体作用机制。方法应用 TCMSP 数据库筛选白芍的有效活性成分，应用 TTD、DrugBank、DisGeNET数据库筛选2型糖尿病相关作用靶点，运用Cytosape 3.2.1软件进行可视化处理及拓扑参数分析，应用 STRING 数据库进行蛋白相互作用分析，应用 DAVID 数据库进行 GO 及 KEGG 通路富集分析。结果白芍抗2型糖尿病的主要活性成分为山柰酚、芍药苷、桦木酸等，其中 BP 主要涉及类固醇激素介导的信号通路、RNA 聚合酶Ⅱ启动子转录的正调控、RNA 聚合酶Ⅱ启动子的转录起始等;CC 涉及细胞器膜、膜、内质网膜等;MF 主要涉及类固醇激素受体、RNA 聚合酶Ⅱ转录因子活性配体激活序列特异性 DNA 结合、锌离子结合等，通路主要涉及糖尿病相关通路有 HIF-1信号通路、 AMPK 信号通路、VEGF 信号通路等。结论白芍可能是通过作用炎症反应、胰岛素抵抗、糖尿病并发症等相关靶点及通路，起到治疗2型糖尿病的作用。

[关键词]网络药理学;白芍;2型糖尿病;作用机制

[中图分类号] R285.5  [文献标识码] A   [文章编号]2095-0616（2021）23-0035-05

A probe into the action mechanism of treating type 2 diabetes with debark peony root based on network pharmacology

JINMeiying1 PANWeiwei2 CUIZhenhai

1.Department of Endocrinology and Metabolic Diseases， the Third Affiliated Hospital of Changchun University of Chinese Medicine， Jilin， Changchun 130117， China;2.College of Traditional Chinese Medicine， Changchun University of Chinese Medicine， Jilin， Changchun 130117， China;3.Orthopedic Center， the Third Affiliated Hospital of Changchun University of Chinese Medicine， Jilin， Changchun 130117， China

[Abstract] Objective To probe into the specific action mechanism of treating type 2 diabetes （T2D） with debark peony root by applying network pharmacology. Methods TCMSP database was used to screen the effective active components of debark peony root. TTD， DrugBank and DisGeNET databases were used to screen the T2D-related action targets. Cytosape 3.2.1 software was used for visualization processing and topological parameter analysis. STRING database was used for protein interaction analysis. DAVID database was used for GO and KEGG pathway enrichment analysis. Results The main active components of debark peony root against T2D were kaempferol， paeoniflorin and betulinic acid， etc.， among which BP mainly involved steroid hormone-mediated signaling pathway， positive regulation of transcription from RNA polymerase Ⅱ promoter， and transcription initiation of RNA polymerase Ⅱ promoter， etc.， CC involved organelle membrane， membranes， endoplasmic reticulum membrane， etc.， MF mainly involved steroid hormone receptor， RNA polymerase Ⅱ transcription factor active ligand-activated sequence-specific DNA binding， zinc ion binding， etc.， and the pathways were mainly diabetes-related pathways such as HIF-1 signaling pathway， AMPK signaling pathway， VEGF signaling pathway， etc. Conclusion It can be concluded that debark peony root may be effective in treating T2D through acting on the relevant targets and pathways of inflammatory response， insulin resistance and diabetic complications， etc.

[Key words] Network pharmacology; Debark peony root; Type 2 diabetes; Action mechanism

糖尿病（diabetes mellitus，DM）是以血中葡萄糖水平升高为生化特征及多饮、多食、多尿、消瘦、乏力为临床特征的代谢紊乱综合群[1]，其中2型糖尿病患病人数占糖尿病患者总数的90%以上[2]。目前中国已成为全球糖尿病患者人数最多的国家[3]，我国18岁以上成年人中2型糖尿病的发病率高达11.6%[4]，2型糖尿病及其并发症严重危害人类健康，给家庭及社会带来巨大经济负担。

2型糖尿病属于中医的“消渴”范畴，《素问·奇病论》：“此人必数食甘美而多肥也，肥者令人内热，甘者令人中满，故其气上溢，转为消渴”[5]。……