周艳霞 罗关靖 普兴宏 戚彦飞

[摘要]目的研究桑生地黃汤对代谢综合征大鼠炎症因子的干预作用。方法 SD 大鼠60只，10只为空白对照组，50只通过高脂高糖高胆固醇饲料饲养制备代谢综合征模型，模型大鼠随机分为5组，每组各10只，分别为模型对照组、二甲双胍组、桑生高剂量组、桑生中剂量组、桑生低剂量组，分别给予相应浓度药物，空白对照组和模型对照组给予同体积的生理盐水，观察各组血糖、FINS、IRI、IL-6、TNF-α水平。结果模型对照组大鼠与空白对照组比较，血糖、IRI、IL-6、TNF-α均明显升高（ P <0.01），桑生高剂量组与模型对照组比较，桑生高剂量组血糖（ P <0.05）、IRI（ P <0.01）、IL-6（ P <0.01）均低于模型对照组，二甲双胍组与模型对照组比较，二甲双胍组血糖、IRI 均低于模型对照组（ P <0.05）。结论二甲双胍组与桑生高剂量组对代谢综合征大鼠各炎症因子有干预作用，且桑生高剂量组效果更优。

[关键词]桑生地黄汤;代谢综合征;动物实验;炎症因子;胰岛素抵抗指数

[中图分类号] R285.5  [文献标识码] A   [文章编号]2095-0616（2021）23-0023-04

Experimental study on the intervention effect of SangshengDihuang Decoction on IRI， IL-6 and TNF-α in rats with metabolic syndrome

ZHOUYanxia1 LUOGuanjing2 PUXinghong2 QIYanfei2

1.Department of Diabetes， Qujing Hospital of Traditional Chinese Medicine， Yunnan， Qujing 655000， China;2.Department of Cardiology， Qujing Hospital ofTraditional Chinese Medicine， Yunnan， Qujing 655000， China

[Abstract] Objective To study the intervention effect of SangshengDihuang Decoction on inflammatory factors in rats with metabolic syndrome. Methods A total of 60 SD rats were selected， of which 10 rats were assigned into the blank control group. The remaining 50 rats were fed with high-fat， high-sugar and high-cholesterol diet to establish the model of metabolic syndrome. The model rats were randomly divided into 5 groups with 10 rats in each group： the model control group， metformin group， Sangsheng high-dose group， Sangsheng middle-dose group and Sangsheng low-dose group. The corresponding concentrations of drugs were given respectively. The blank control group and the model control group were given the same volume of normal saline. The levels of blood glucose， fasting insulin （FINS）， ischemia-reperfusion injury （IRI）， interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） were observed in each group. Results Compared with those in the blank control group， the blood glucose， IRI， IL-6 and TNF-α in the model control group increased significantly （P <0.01）. The levels of blood glucose （P <0.05）， IRI （P <0.01） and IL-6（P <0.01） in the Sangsheng high-dose group were lower than those in the model control group. The levels of blood glucose and IRI in the metformin group were lower than those in the model control group （P <0.05）. Conclusion The metformin group and Sangsheng high-dose group shows intervention effect on inflammatory factors in the rats with metabolic syndrome， and Sangsheng high-dose group exhibits better effect.

[Key words] SangshengDihuang Decoction; Metabolic syndrome; Animal experiment; Inflammatory factors; Insulin resistance index

代谢综合征是以向心性肥胖为特征的2型糖尿病与心血管疾病危险因素的集合体。在我国代谢综合征患病率逐年增高，且年轻化趋势明显，成为威胁人们身体健康的巨大隐患。代谢综合征人群的减重、改善胰岛素抵抗，是有效预防2型糖尿病和心血管疾病的重要手段。桑生地黄汤为我科长期使用有效的自拟方剂，对肥胖型2型糖尿病患者有明显降糖、减重作用[1]，对代谢综合征大鼠脂肪因子有明显调节作用[2]。本研究主要通过桑生地黄汤对代谢综合征大鼠胰岛素抵抗指数（insulin resistance index，IRI）、白细胞介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α， TNF-α）三个指标的干预效果来观察桑生地黄汤改善胰岛素抵抗、减轻微炎症反应的作用，以进一步探讨桑生地黄汤对代谢综合征大鼠的相关作用机制。

1材料与方法

1.1实验动物

雄性 SD 大鼠60只，SPF 级，6～8周，体重180～220 g，成都科硕实验动物中心提供，许可证号：dy20190402，饲养于 SPF 屏障系统。……