汪艳平 韦正 蒋利荣 张鹏 陈海林 符益山 覃拥灵

摘要 [目的]运用网络药理学反向分子对接的原理，对山豆根抑癌作用机制进行预测。[方法]筛选山豆根化学成分25个，通过PharmMapper数据库查找相关靶点，NCBI数据库进行靶点的校正，利用DAVID数据库筛选药物的KEGG通路，运用cytoscape建立山豆根的“药材-化学成分-靶点-作用通路-药理作用-临床应用”多层次作用网络图。[结果]山豆根的25个化学成分可调控CTBP2、CDKN1B、ABL1等51个靶点，得到相关通路25条，涉及癌症、炎症、肝病、心血管病等疾病，其中10条通路与癌症相关。[结论]通过网络药理学的分析方法，预测了山豆根的抗癌作用机制，为深入研究山豆根的药理作用提供方向。

关键词 山豆根;网络药理学;抗癌;作用通路

中图分类号 R285 文献标识码 A

文章编号 0517-6611（2021）17-0165-05

doi：10.3969/j.issn.0517-6611.2021.17.044

Abstract [Objective] To predict the antitumor mechanism of Sophora tonkinensis by using the principle of reverse molecular docking in network pharmacology.[Method]25 chemical constituents of Sophora tonkinensis were screened， and the related targets were searched through the pharmapper database， the targets were adjusted by NCBI database， the KEGG pathway of drugs was screened by DAVID database， and the multi-level action network diagram of “medicinal materials-chemical composition-target-action pathway-pharmacological action-clinical application” was established by using Cytoscape.[Result]The 25 chemical components of Sophora tonkinensis could regulate 51 targets such as CTBP2， CDKN1B and ABL1， and 25 related pathways were obtained， involving cancer， inflammation， liver disease， cardiovascular disease and other diseases.Ten of them are related to cancer.[Conclusion]The anti-cancer mechanism of Sophora tonkinensis is predicted by network pharmacology analysis method， which provides direction for further study on pharmacological action of Sophora tonkinensis.

Key words Sophora tonkinensis;Network pharmacology;Anti-cancer;Action pathway

山豆根是豆科植物越南槐（Sophora tonkinensis Gagnep）的根，又名苦豆根（《中藥材手册》）、广豆根、小黄连、三小叶山豆根、胡豆莲、柔枝槐等，《经验方》中记载其为黄结[1]。山豆根主要化学成分有苦参碱、氧化苦参碱、金雀花碱和甲基金雀花碱等生物碱;山豆根色满二氢黄酮、槲皮素、芦丁等黄酮类;羽扇豆醇、槐花二醇等三萜类和小分子酚性及其他化学成分，如麦芽酚、大黄素、β-谷甾醇、三叶豆紫檀苷等。2015年版《中华人民共和国药典》中对山豆根记载是“有豆腥气，味极苦，性寒，归肺、胃经”。《本草图经》中记载山豆根含服可治咽喉肿痛;《本草纲目》曰：“清肺热，除痰咳”。现代药理研究表明，山豆根主要有抑癌、抑菌、抗炎、抗病毒、抗心律失常等药理活性[2-3]。

山豆根的抗肿瘤作用越来越受到人们的关注。……