杨祎辰 王二欢 王继强 马存德 常晖 靳鹏博

摘要 [目的]通过测定不同产地黄芪的主要活性成分含量，研究不同成分间的相互关联性。[方法]收集甘肃陇西及周边共7个产地36批次黄芪样品，分别测定总糖、还原糖、总黄酮、毛蕊异黄酮葡萄糖苷、总皂苷和黄芪甲苷6种主要活性成分，对测定数据进行Pearson相关性分析并建立回归方程。[结果]36批次黄芪样品的黄芪甲苷和毛蕊异黄酮葡萄糖苷含量基本符合药典标准。黄芪的主要活性成分间呈现出一定的相关性。其中，还原糖与总糖呈极显著正相关，毛蕊异黄酮葡萄糖苷与总黄酮呈显著正相关，黄芪甲苷与还原糖呈显著负相关。[结论]该研究为进一步探讨甘肃道地药材黄芪与自然生态环境的相关性提供数据支撑和理论依据。

关键词 黄芪;道地药材;活性成分;相关分析;不同产地;甘肃

中图分类号 R282 文献标识码 A

文章编号 0517-6611（2021）17-0161-04

doi：10.3969/j.issn.0517-6611.2021.17.043

Abstract [Objective]By measuring the content of the main active ingredients of Astragalus membranaceus from different origins，the correlation between them was studied.[Method]A total of 36 batches of Astragalus membranaceus samples from 7 producing areas in Longxi of Gansu and its surrounding areas were collected，the 6 main active components of total sugar，reducing sugar，total flavonoids，calycosin-7-glucoside，total saponins and astragaloside IV were determined，pearson correlation analysis of the measured data was performed and the regression equation was established.[Result]The contents of astragaloside IV and calycosin-7-glucoside in 36 batches of Astragalus membranaceus samples met the pharmacopoeia standards.The main active components of Astragalus membranaceus showed a certain correlation.Among them，reducing sugars had a very significant positive correlation with total sugars，calycosin-7-glucoside had a significant positive correlation with total flavonoids，and astragaloside IV had a significant negative correlation with reducing sugars.[Conclusion]This research provides data support and theoretical basis for further exploring the correlation between the authentic medicinal material Astragalus membranaceus in Gansu and the natural ecological environment.

Key words Astragalus membranaceus;Famous-region drug; Active ingredients; Correlation analysis;Different origins;Gansu

黃芪，亦作“黄耆”，始载于《神农本草经》。李时珍释其名曰：“耆，长也，黄耆色黄，为补药之长，故名”。黄芪资源分布广，种植历史悠久，当前，甘肃为我国黄芪主产区之一。陶弘景言“虚而冷，用陇西黄芪”，可见陇西产黄芪自古得名[1]。陇西县位于甘肃省东南部，渭河上游，海拔1 612～2 778 m，为典型的黄土梁峁与河谷地形，四季分明，日照充足，气候温和，其气候特点与黄芪生长发育规律相吻合。因而素有“千年药乡”之称，享有“西北药都”“中国黄芪之乡”等盛誉，陇西黄芪为国家地理标志产品，陇西亦为黄芪的道地产区之一。……