姚铭榕 谢凯玲 张文静 张文杰 史庆华 刘杰

摘    要：为研究2个番茄品种WK53、WK54的耐盐性，采用200 mmol·L-1 NaCl处理幼苗，分别测定其生长指标、可溶性糖、Na+、K+含量，以及抗盐基因SOS1、SOS2相对表达量。结果表明，盐胁迫下，2个品种番茄展叶数、株高、茎粗的相对增长值均受抑制;可溶性糖含量较对照组分别增加了66.1%、73.7%;而叶片Na+、K+含量却只有少量提高;抗盐基因SOS1、SOS2相对表达量均受盐胁迫诱导，尤其是在WK54中，SOS2表达水平较对照组上升18倍。综合分析表明，在幼苗期WK53、WK54均具有一定的耐盐性，但WK54的耐盐性更好，SOS2的高表达可能是WK54更耐盐的关键因素。

关键词：番茄;盐胁迫;生长指标;耐盐基因

中图分类号：S641.2 文献标志码：A 文章编号：1673-2871（2021）08-021-05

Evaluation of salt tolerance of two tomato varieties

YAO Mingrong1，2， XIE Kailing3， ZHANG Wenjing3， ZHANG Wenjie3， SHI Qinghua1， LIU Jie2，3

（1. College of Horticulture Science and Engineering， Shandong Agricultural University， Taian 271018， Shandong， China; 2. Facility Horticulture Laboratory of Universities in Shandong/Weifang University of Science and Technology， Shouguang 262700， Shandong， China; 3. School of Agronomy and Environment， Weifang University of Science and Technology， Shouguang 262700， Shandong， China）

Abstract： In order to study the salt tolerance of two tomato varieties WK53 and WK54， the seedlings were treated with 200 mmol·L-1 NaCl， and their growth indexes， soluble sugar content， Na+ content， K+ content and the expression levels of salt-resistant genes SOS1 and SOS2 were determined. The results showed that the relative increments in leaf number， plant height and stem diameter were inhibited in two varieties under salt stress; the contents of soluble sugar increased by 66.1% and 73.7% than these in control; however， the contents of Na+ and K+ in leaves increased slightly; the expression levels of salt-resistant genes SOS1 and SOS2 were induced under salt stress. Compared with the control， the expression level of SOS2 increased 18 times under 200 mmol·L-1 NaCl treatment in WK54. Comprehensive analysis shows that both WK53 and WK54 are salt-tolerant to a certain extent， but WK54 has a higher salt tolerance at the seedling stage. The high expression of SOS2 may be the key factor for WK54 to be more salt tolerant.

Key words： Tomato; Salt stress; Growth index; Salt tolerance gene

壽光市被誉为“蔬菜之乡”“海盐之都”，位于山东半岛中北部，渤海莱州湾西南岸，其北部地区潜水矿化度高，土层含盐量大，土壤盐渍化问题严重，属于滨海湿润-半湿润海侵盐渍区;而南部地区虽然有较为良好的土壤条件，但是由于近年来设施蔬菜发展迅速，长年集约化经营引起土壤酸化与盐渍化，极大地影响了蔬菜的产量和品质[1-2]。提高植物耐盐性，培育和筛选耐盐蔬菜品种是有效利用盐渍化土壤的途径之一。

番茄（Solanum lycopersicon）属中等耐盐碱蔬菜作物[3]，其适应性强，是世界栽培历史悠久、栽培地区广泛的重要果菜种类，也是我国设施蔬菜栽培面积和消费量最大的蔬菜作物之一。目前，对于番茄品种耐盐性的研究大多为单一的生理生化或分子改良方面的研究[2，4-6]，而将其苗期的生理生化与相关抗盐基因表达情况相结合来评价其耐盐性未见报道。……