两种生长阶段的牛蛙蝌蚪对盐度的耐受性研究
2021-09-03梁家豪鲜雪梅
梁家豪 鲜雪梅



摘要 在人工养殖条件下,盐度常作为影响蝌蚪生长发育的环境因子之一。在实验室温度(23.1±2.0) ℃环境下,采用单因子静态急性毒性试验法研究2种生长期牛蛙 (Rana catesbeiana Shaw)蝌蚪对水体盐度的耐受能力。结果表明,在试验条件水质下,处于25期牛蛙蝌蚪及五趾分化期牛蛙蝌蚪的24、48、72和96 h半致死浓度分别为6.41、5.44、5.50、5.37 g/L及12.56、10.48、7.67、4.09 g/L,安全值分别为1.18 和2.19 g/L。在相同条件下,五趾分化期牛蛙蝌蚪比25期牛蛙蝌蚪对盐度的耐受能力更强。建议在人工养殖过程中,将25期牛蛙蝌蚪及五趾分化期牛蛙蝌蚪分池饲养,二者长期生存的安全盐度控制在1.3 g/L以下。
关键词 牛蛙蝌蚪;半致死浓度;盐度;耐受性;安全值
中图分类号 S 917.4 文献标识码 A
文章编号 0517-6611(2021)14-0085-04
Abstract Under the conditions of artificial culture,salinity was often used as one of the environmental factors that affected the growth and development of tadpoles.The tolerance to salinity of tadpoles of Rana catesbeiana Shaw at two growth stages was studied by using a singlefactor static acute toxicity test under the laboratory environment with an average temperature of (23.1±2.0)℃.The results showed that under the experimental conditions,24,48,72 and 96 h LC50 of R.catesbeiana tadpoles at the 25 stage and 5toed differentiation stage were 6.41,5.44,5.50,5.37 and 12.56,10.48,7.67,4.09 g/L respectively,SC value was 1.18 and 2.19 g/L respectively.Under the same conditions,the tolerance to salinity of R.catesbeiana tadpole at 5toed differentiation stage was stronger than that of bullfrog tadpole at the 25 stage.It was suggested that in the process of artificial breeding, R.catesbeiana tadpoles at 25stage and 5toed differentiation stage should be fed in separate ponds,and the safe salinity of their longterm survival should be controlled below 1.3 g/L.
Key words Rana catesbeiana tadpoles;Half lethal concentration;Salinity;Tolerance;SC
基金項目 阿坝师范学院青年基金项目(ASC20-04);国家级大学生创新训练项目(202010646031);2019年学生科研项目(201910089,201910099);阿坝师范学院质量工程项目(201909023)。
作者简介 梁家豪 (1996—),男,四川西昌人,研究方向:动物生理生态。*通信作者,讲师,从事动物生理生态研究。
收稿日期 2020-12-23
牛蛙 (Rana catesbeiana Shaw)属于两栖纲(Amphibia)无尾目(Anura)蛙科(Ranidae)[1],体型较大。牛蛙肉味道非常鲜美,且肉质细腻嫩滑,含有丰富的营养元素,具有较高的药用价值。牛蛙肉不仅可以食用,而且可以药用调理,蛙皮也能供人使用,可以说是名副其实的“宝藏蛙”。牛蛙肉可作为上等美味食材和高级的养生药膳,是一种蛋白质含量高、脂肪和胆固醇含量低的健康肉类。牛蛙肉的药用价值也非常高,具有滋补解毒的功效[2]。对于做完手术后需要营养恢复的伤者来说,牛蛙肉可以促进伤口的愈合。……
