唐靖雯 潘梅 张丽艳 梁斌 卢礼平 潘婷

【摘 要】 目的：确定花菊盆炎颗粒最佳提取工艺。方法：应用HPLC测定槲皮素含量，并与正丁醇浸出物总量的综合评分为考察指标，采用正交试验法L9（34）优选提取工艺条件。结果：最佳提取工艺为处方中头花蓼、醋延胡索、三七加7倍量60%乙醇提取3次，每次1.5 h，过滤得醇提液;野菊花、夏枯草、茜草炭、粉萆薢加10倍量的水煎煮3次、每次1 h，滤过，滤液浓缩至相对密度为1.10-1.15（60 ℃）的浸膏，放冷，加乙醇使药液含醇量达70%，静置后过滤，得水提醇沉液;合并上述滤液，回收乙醇并浓缩至相对密度为1.20-1.25（60 ℃）。结论：该工艺简单、稳定可行，对花菊盆炎颗粒的工业化生产具有一定的参考价值。

【关键词】 花菊盆炎颗粒;正交实验法;醇提法;水提醇沉法

【中图分类号】R284 【文献标志码】 A 【文章编号】1007-8517（2021）12-0000-00

Abstract：Objective To optimum the extraction procedure of Huaju Penyan granules. Methods The content of quercetin was determined by HPLC，and the comprehensive score of the total amount of n-butanol extract was used as the index. The orthogonal test method L9（34） was used to optimize the extraction process conditions. Results The best extraction process was to extract Polygonum capitatum，Rhizoma Corydalis （made by vinegar） and Panax notoginseng with 7 times of 60% ethanol for 3 times，1.5 h each time，and then filtered to obtain the alcohol extract; Chrysanthemum indicum，Prunella vulgaris，cypermethrin and were decocted with 10 times amount of water for 3 times，each time for 1 h. After filtration，the filtrate was concentrated to the extract with a relative density of 1.10-1.15 （60 ℃），cooled，added with ethanol to make the alcohol content of the solution reach 70%. After standing and filtering，water extraction and alcohol precipitation were obtained; The above filtrate was combined，ethanol was recovered and concentrated to a relative density of 1.20-1.25 （60 ℃）. Conclusion The optimum extraction procedure is simple，stable and practical，which has a certain reference value for the industrial production of Huaju Penyan granules.

Keywords：Huaju Penyan Granules; Qrthogonal Test; Alcohol Extraction; Water Extracting-alcohol Precipitating

花菊盆炎顆粒是利用贵州苗药头花蓼优良的抗菌消炎活性特点，并结合中医药理论及名老中医的临床治疗经验，采用头花蓼为君药，配以野菊花、夏枯草、醋延胡索、茜草炭、三七、粉萆薢等六味中药组成经验方;由贵州威门药业股份有限公司自主研发的一种治疗女性慢性盆腔炎的纯中药复方制剂，属于国家6类中药新药。头花蓼中主要含有芦丁、槲皮素等黄酮类成分，溶于乙醇，具有明显的消炎、抗菌作用[1];野菊花中主要含有萜类化合物、黄酮类化合物等，水提液具有明显的抗菌、抗病毒、抗抑郁和消炎等作用[2];夏枯草的主要成分为三萜及其苷类、苯丙素类、黄酮类、香豆素等，其水提……