苹果蠹蛾雄虫发生动态及其与气温和降水的相关性研究
2021-07-25李平
李平



摘要:采用系统监测的方法调查研究了2018 — 2020年甘肃省武威市凉州区梨树上苹果蠹蛾雄虫种群发生动态及其与气候因子的相关性。结果表明,金武地区苹果蠹蛾1 a发生2代,雄虫的月诱虫总量(y)与月气温总量(x)之间呈显著正相关,回归模型方程为y=0.1947x-58.967(R2=0.807 6);苹果蠹蛾雄虫的月诱虫总量(y)与月降水总量(z)之间无显著相关性。
关键词:苹果蠹蛾;雄虫;种群发生动态;气温;降水量
中图分类号:S436.611.2 文献标志码:A 文章编号:1001-1463(2021)06-0052-03
doi:10.3969/j.issn.1001-1463.2021.06.013
Molecular Identification of 21 Strains of Wild Morchella spp. from Gannan Tibetan Autonomous Prefecture
YANG Qin 1, WANG Sanxi 2, WANG Haifeng 2, YE Xiaoyan 3
(1. Institute of Vegetable, Gansu Academy of Agricultural Sciences, Lanzhou Gansu 730070, China;2. Agricultural Science Research Institute of Gannan Tibetan Autonomous Prefecture, Gannan Gansu 747000, China;3. College of Life Science, Northwest Normal University, Lanzhou Gansu 730070, China)
Abstract:Gannan Tibetan Autonomous Prefecture has high cold damp, large temperature difference between day and night, and fertile soil, which is very suitable for the growth of Morchella spp. 21 mycelium cultures were obtained by tissue isolation from wild Morchella collected from Gannan Tibetan Autonomous Prefecture in this study, and were identified by ITS, ef1-α, rpb1 and rpb2 joint matrix sequence analysis. The results showed that all the 21 strains were Elata Clade, 19 of which were Morchella eohespera, and 2 strains were Morchella spp. Mel-13., Moreover, these two kinds of Morchella have been cultivated artificially.
Key words:Gannan Tibetan Autonomous Prefecture;Morchella spp.;Molecular identification;Sequence joint analysis
苹果蠹蛾(Cydia pomonella L.)是对水果生产都有重大影响的重要害虫[1 - 2 ],其幼虫蛀果为害、食性杂、环境适应力强[3 - 4 ], 严重影响果品的品质和产量, 常常造成毁灭性危害, 我国将其列入农业、林业检疫性有害生物及进境植物检疫性有害生物名 录[1 ]。特色林果业是目前甘肃省金武地区的重要产业之一,苹果蠹蛾是威胁甘肃河西果业生产的重大害虫。但金武地区苹果蠹蛾种群发生动态及其与气候因子的相关性研究鲜有报道。笔者于2018 — 2020年在甘肃省武威市凉州区设立了苹果蠹蛾监测点,系统调查和研究了苹果蠹蛾雄虫发生动态及其与气温、降水的相关联系,旨在为金武地区苹果蠹蛾的科学防治和预测预报提供参考。
1 材料与方法
1.1 供试材料
供试橡皮头式苹果蠹蛾长效性信息素诱芯由北京中捷四方生物科技股份有限公司生产提供,诱捕器为自制苹果蠹蛾捕捉器(专利号:ZL 2016 2 0453678.X)。监测对象为苹果蠹蛾(Cydia pomonella L.)雄虫,寄主作物为梨树,品种为皇冠梨。
1.2 試验方法
监测地点位于甘肃省武威市凉州区高坝镇楼庄村。……
