刘启月 李勇 陈小龙 余向阳

摘要： 為深入了解桃胶的营养价值，首先分析了5种不同颜色桃胶提取液中总酚含量及抗氧化活性差异，然后基于高效液相色谱-四极杆飞行时间质谱（LC-QTOF-MS）和高效液相色谱串联质谱（LC-MS）的代谢组学方法对桃胶提取液中的酚类物质进行定性定量分析。结果表明，不同颜色桃胶提取液中总酚含量和抗氧化活性存在明显差异，颜色越深总酚含量越多、抗氧化活性越强，颜色最深样品（S5）中总酚含量为28.23 μmol/g，是颜色最浅样品组的7.8倍。基于LC-QTOF-MS分析桃胶提取液，共鉴定出32种活性化合物，其中黄酮类化合物有20种;基于LC-MS的靶向代谢组学方法对18种酚类化合物进行定量分析，发现橙皮素、柚皮素、表儿茶素、圣草酚含量最多。相关性分析结果表明，18种酚类化合物中，14种酚类化合物与总酚含量及抗氧化活性呈显著正相关关系。

关键词： 桃胶;酚类化合物;抗氧化活性;代谢组学

中图分类号： TS218 文献标识码： A 文章编号： 1000-4440（2021）03-0746-08

Analysis on phenolics contents and antioxidant activity in peach gum based on metabolomics

LIU Qi-yue1，2，3， LI Yong2，3， CHEN Xiao-long2，3， YU Xiang-yang1，2，3

（1.College of Food Science and Engineering， Nanjing University of Finance and Economics， Nanjing 210023， China;2.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Constructed by Province-Ministry， Nanjing 210014， China;3.Institute of Agricultural Resources and Environment， Jiangsu Academy of Agricultural Sciences， Nanjing 210014， China）

Abstract： To better understand the nutritive value of peach gum， total phenol content and antioxidant activity difference in the extracting solution of peach gum presenting five different colors were analyzed firstly. Then the phenolic substances in the extracting solution of peach gum were analyzed qualitatively and quantitatively via metabolomics methods of liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry （LC-QTOF-MS） and liquid chromatograph-mass spectrometer （LC-MS）. The results showed that， total phenol content and antioxidant activity of peach gum extracting solutions with various colors were significantly different， and the deeper the color， the higher the total phenol content was， with stronger antioxidant activity. The total phenol content in the peach gum with the darkest color （S5） was 28.23 μmol/g， which was 7.8 times compared with the lightest color group. 32 active metabolites were identified in the extracting solution of peach gum via LC-QTOF/MS methods， including 20 flavonoid compounds. Quantitative analysis of 18 phenol compounds based on target metabonomics of LC-MS showed that， the contents of hesperetin， naringenin， epicatechin and eriodictyol were relatively high. Correlation analysis showed that， 14 phenolic compounds of 18 phenolic compounds in peach gums showed significant positive correlation with total phenols content and antioxidant activity.

Key words： peach gum;phenolic compounds;antioxidant activity;metabolomics

桃胶为蔷薇科植物桃[Prunus persica （L.） Batsch]或山桃[Prunus davidiana （Carr.） Franch]等植物的树皮受损后分泌出来的半透明无色、浅黄色或黄棕褐色琥珀状固体，初分泌时为透明状黏液，随着风干日晒，经氧化浅层变黑后透明度降低。古籍记载，桃胶味苦、平、无毒，炼服能保中不饿、和血益气、下石淋、止痛等[1]。桃胶可活血消肿、通淋止痛，临床运用对腹泻、疼痛、血淋、石淋、痢疾等症均有效，被《中药大辞典》收录[2]。桃胶70%～80%的成分为桃胶多糖，现代医学研究结果表明，桃胶多糖具有良好的体内外抗氧化活性[3]，且能提高免疫力、降血糖、降血脂、调节胃肠道[4-5]。……