张雨凝 何敏 韦靖婧 郭熙艳

摘要： 为探索茜草制诃子对免疫抑制中国黄羽鹌鹑法氏囊T-bet及GATA-3表达量的影响，以茜草制诃子为研究对象，选用160羽21日龄健康中国黄羽鹌鹑，随机分为8组，进行临床观察、苏木精-伊红（HE）染色、荧光定量PCR等试验。临床观察结果显示，诃子组及茜草制诃子组平均日增质量增幅较空白组略小，但较阴性组增幅明显较大。各组法氏囊HE染色结果显示，诃子组及茜草制诃子组相较于空白组淋巴滤泡面积减小、法氏囊皱襞高度降低，但均优于阴性组。T-bet和GATA-3表达量趋势均为空白组>茜草制诃子2 g/kg组>诃子1 g/kg组>茜草制诃子4 g/kg组>茜草制诃子1 g/kg组>诃子2 g/kg组>诃子4 g/kg组>阴性组。说明，诃子及茜草制诃子均对免疫抑制中国黄羽鹌鹑法氏囊的免疫功能有恢复作用，茜草制诃子治疗效果优于诃子。研究结果为探索关于治疗免疫抑制疾病、提高動物自身免疫功能的药物提供了一些理论依据。

关键词： 茜草制诃子;免疫抑制;中国黄羽鹌鹑;法氏囊

中图分类号： R285.5 文献标识码： A 文章编号： 1000-4440（2021）03-0704-06

Effects of Rubia cordifolia L. per Terminalia chebula on expression of T-bet and GATA-3 in bursa of Fabricius of Chinese Yellow Quail

ZHANG Yu-ning1，2， HE Min1，2， WEI Jing-jing1，2， GUO Xi-yan1，2

（1.College of Veterinary Medicine， Sichuan Agricultural University， Chengdu 611130， China;2.Key Laboratory of Animal Diseases and Human Health of Sichuan Province， Chengdu 611130， China）

Abstract：To explore the effect of Rubia cordifolia L. per Terminalia chebula on the expression of T-bet and GATA-3 in bursa of Fabricius of immunosuppressed Chinese Yellow Quail， Rubia cordifolia L. per Terminalia chebula was used as the research object， 160 21-day-old Chinese Yellow Quails were randomly divided into eight groups to do clinical observation， hematoxylin-eosin （HE） staining， fluorescence quantitative PCR and other experiments. Results of clinical observation showed that， the daily weight gains of Terminalia chebula group and Rubia cordifolia L. per Terminalia chebula group were slightly lower than that of blank group， but were obviously higher than that of negative group. HE staining results of bursa of Fabricius showed that， compared with the blank group， the area of lymphoid follicle and the height of Fabricius plica of Terminalia chebula group and Rubia cordifolia L. per Terminalia chebula group decreased， but they were improved compared with the negative group. The expression trends of T-bet and GATA-3 were blank group> Rubia cordifolia L per Terminalia chebula 2 g/kg group> Terminalia chebula 1 g/kg group > Rubia cordifolia L. per Terminalia chebula 4 g/kg group> Rubia cordifolia L. per Terminalia chebula 1 g/kg group> Terminalia chebula 2 g/kg group> Terminalia chebula 4 g/kg group> negative group. The results show that both Terminalia chebula and Rubia cordifolia L. per Terminalia chebula can recover the immunosuppressed bursa of Fabricius of Chinese Yellow Quail， and compared with Terminalia chebula， Rubia cordifolia L. per Terminalia chebula has a better effect. It provides some theoretical basis for the exploration of drugs for treating immunosuppressive diseases and improving animal autoimmunity.

Key words： Rubia cordifolia L. per Terminalia chebula;immunosuppression;Chinese Yellow Quail;bursa of Fabricius

茜草（Rubia cordiforlia L.）为植物茜草的干燥根和茎[1]。诃子（Terminalia chebula）为使君子科植物诃子或绒毛诃子的干燥成熟果实[2]。诃子作为主药、茜草作为辅药的配伍炮制方法在藏药方剂中早有记录[3]，两者可相辅相成。中国黄羽鹌鹑（Chinese Yellow Quail）是经选育培养成功的品种，因其生命力强、生长快、抗病性强等特点，在育种、生产、经济等方面都具有很高的价值[4]。法氏囊（Bursa of Fabricius， BF）作为禽类特有的中枢免疫器官，现有研究主要集中于其结构与功能发育、免疫功能的调控以及传染性法氏囊病[5]。虽然人们已对禽类广泛接种各类……