彭凌 林锦铨 李玲慧 刘博婷 蔡巩林

摘要： 本研究测定猪丹毒丝菌临床毒株SG7的全基因组序列，并运用生物信息学方法对测定的全基因组序列及猪丹毒丝菌表面保护性抗原A基因（SpaA）进行分析。SG7菌株的基因组全长为1 834 291.00 bp，G+C含量为36.3%，基因总数1 846个。将SG7菌株与GenBank中8条完整的猪丹毒丝菌全基因组序列进行比较，发现国内外不同菌株间基因组的基本信息存在不同程度差异。基于全基因组单核苷酸多态性（SNPs）的系统发育分析结果表明，9株菌株聚为3个分支，国内菌株并不完全处于同一分支，SG7菌株与国内常见菌株不属于同一进化分支。SpaA基因高变区的分析结果显示，9株菌株亦可分为3个SpaA型，SG7菌株为携带Met203的高致病性菌株。除SG7菌株外，其他8株菌株的SpaA基因分型结果与基于全基因组SNPs分析的结果一致，说明SG7菌株的遗传背景复杂。本研究结果可为猪丹毒丝菌基因组整体水平研究和疫苗的研发奠定基础。

关键词： 猪丹毒丝菌;全基因组测序;表面保护性抗原A（SpaA）

中图分类号： S852.61 文献标识码： A 文章编号： 1000-4440（2021）03-0694-05

Whole-genome sequencing and SpaA gene analysis of a Erysipelothrix rhusiopahiae strain

PENG Ling， LIN Jin-quan， LI Ling-hui， LIU Bo-ting， CAI Gong-lin

（Henry Fok College of Biology and Agriculture， Shaoguan University， Shaoguan 512005， China）

Abstract： In this study， the whole genome sequence of Erysipelothrix rhusiopahiae clinical strain SG7 was determined， and the whole genome sequence and the surface protective antigen A （SpaA）gene were analyzed using bioinformatics method. The full length of SG7 genome was 1 834 291.00 bp， and the total quantity of the predicted genes were 1 846 with a G+C content of 36.3%. Different degrees of differences of genomic basic information between different strains were found by comparing the complete genome sequences of SG7 and eight E. rhusiopahiae found in GenBank. Phylogenetic analysis based on genome-wide single nucleotide polymorphisms （SNPs） revealed that nine strains could be clustered into three distinct clades， and strains isolated from China were not in the same clade， while SG7 and common strains isolated from China did not belong to the same clade. The analysis results of hypervariable region of SpaA showed that nine strains could also be divided into three SpaA types， and SG7 was a highly pathogenic strain carrying Met203. Except for SG7， the SpaA genotyping results of other eight strains were consistent with the analytic results obtained based on the genome-wide SNPs analysis， which indicated that the genetic background of SG7 was complex. The results can provide data basis for the research at the whole genome level and development of E. rhusiopahiae vaccine.

Key words： Erysipelothrix rhusiopahiae;whole-genome sequencing;surface protective antigen A （SpaA）

豬丹毒丝菌（Erysipelothrix rhusiopahiae）又称红斑丹毒丝菌，属于丹毒丝菌属（Erysipelothrix），呈纤细杆状，是一种革兰氏阳性的人畜共患病病原菌。猪丹毒是由猪丹毒丝菌引发的一种急性、热性传染病，能导致家禽、畜类的一系列皮肤感染及败血症。人类因外伤局部感染猪丹毒丝菌，会发生类丹毒，感染时手指出现斑点或肿起[1-2]。在20世纪80至90年代，猪丹毒与猪肺疫、猪瘟并称中国养猪业三大传染病，给养猪业带来巨大的损失并影响人类健康[3]。……