切花红掌组培繁殖技术研究
2021-07-16束晓春张彬陆波王忠
束晓春 张彬 陆波 王忠



摘要 [目的]建立切花红掌组织培养快繁体系。[方法]选用切花红掌品种“佛莱”为试材,研究不同外植体、激素对其组织培养诱导、分化和生根的影响。[结果]适宜红掌“佛莱”建立无菌系的外植体为4月10日的顶芽组织;初代诱导培养基为MS+6-BA 1.0 mg/L +NAA 0.3 mg/L;继代培养基为MS+6-BA 0.8 mg/L+NAA 0.3 mg/L,增殖系数达19.4倍;生根培养基为1/2MS +IBA 0.5 mg/L,生根率100%。[结论] 该研究建立的红掌“佛莱”组织培养快繁体系可为切花红掌工厂化生产提供技术参考。
关键词 红掌;组织培养;外植体;顶芽
中图分类号 S-682.1+4 文献标识码 A
文章编号 0517-6611(2021)11-0121-03
doi:10.3969/j.issn.0517-6611.2021.11.034
开放科学(资源服务)标识码(OSID):
Study on in vitro Propagation of Anthurium andraeanum ‘Floris
SHU Xiao-chun1, ZHANG Bin2, LU Bo3 et al
(1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu 210014; 2. Patent Exa mination Collaboration (Beijing) Center of the Patent Office, CNIPA, Beijing 100160; 3.NAN JING LI DAO Modern Agriculture Co., Ltd., Nanjing, Jiangsu 210043)
Abstract [Objective]The research aimed to establish a rapid prapagation system of Anthurium andraeanum. [Method]Taking Anthurium andraeanum ‘Floris as test material, the effects of different explants and hormones on the induction, differentiation and rooting of tissue culture were studied. [Result]The suitable explants for establishing aseptic line of Anthurium andraeanum ‘Floris were the apical bud tissue on April 10, the initial induction medium was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L, the medium of subculture micropropagation was MS+6-BA 0.8 mg/L+NAA 0.3 mg/L on which reproductive rate reached 19.4, and 1/2MS+IBA0.5 mg/L was the rooting medium on which rooting rate reached 100.00%. [Conclusion]The suitable tissue culture formula of Anthurium andraeanum ‘Floris was selected, which can provide the technical support for large scale breeding.
Key words Anthurium andraeanum;Tissue culture;Explant;Terminal bud
紅掌(Anthurium andraeanum),又名花烛、安祖花、火鹤花等,天南星科花烛属多年生草本植物,原产于南美,喜温暖湿润气候,因花型独特,佛焰苞鲜艳亮丽,色彩丰富,花期长,叶形别致,观叶观花俱佳,已成为仅次于热带兰的热带花卉种类,在我国也比较流行[1-2]。近几年,荷兰AVO公司推出的切花红掌系列新品种因其花大色艳,尤其受到欢迎,市场供不应求,成为华北、华东地区重要的设施栽培切花花卉。该类品种多为国外引进,种苗数量有限,大面积推广应用受到限制。红掌不易结实,一般采用分株繁殖[3],但其肉质根系生长缓慢、分蘖较少,繁殖率极低,用分株繁殖难以扩大生产,故常规繁殖法难以满足市场需求,而组织培养被认为是园艺商品生产中快速繁殖的理想途径。因此,市场推广应用亟需组培快繁技术体系的建立。
目前,有关于红掌种苗繁殖已有不少报道,其中亦有组培繁殖技术方面的研究[4-7],然而,肖三元等[8-9]研究发现,由于植物不同品种间不同外植体的脱分化能力存在一定差异,以往的研究方法不适用于“佛莱”(Floris)等新品种的繁殖。……
