王晓丹 郭星汉 崔瑛

摘要 目的：观察细辛水煎液和挥发油混合物对利血平诱导抑郁模型大鼠的抗抑郁作用并探讨其作用机制。方法：除正常对照组外，其余各组大鼠每天给予利血平腹腔注射，造模第4天各组大鼠开始灌胃给药，连续给药12 d。记录大鼠的体质量、肛温及敞箱实验大鼠运动距离、站立次数变化。采用酶联免疫吸附试验法（ELISA）测定单胺类递质及其代谢产物的含量。结果：与正常对照组比较，模型组大鼠体质量、肛温、敞箱运动距离与站立次数均显著降低，差异有统计学意义（P<0.05），脑组织中5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）含量显著减少，差异有统计学意义（P<0.05）。与模型组比较，细辛水煎液和挥发油混合物高、中、低剂量组均能够不同程度的纠正利血平所致大鼠体质量、肛温、敞箱运动距离与站立次数降低的趋势，增加大鼠脑组织中5-HT、DA、NE的含量，降低大鼠腦组织中5-羟吲哚乙酸（5-HIAA）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）的含量。结论：细辛水煎液和挥发油混合物在利血平诱导抑郁大鼠模型上表现出较好的抗抑郁作用，其抗抑郁作用的发挥可能与增强单胺类递质的合成，同时抑制单胺类递质的代谢密切相关。

关键词 细辛;抑郁;机制;单胺类递质

Effects of Mixture of Xixin Decoction and Volatile Oil on Reserpine-induced Depression in Rats

WANG Xiaodan，GUO Xinghan，CUI Ying

（Henan University of Chinese medicine，Henan，Zhengzhou 450046，China）

Abstract Objective：To observe the antidepressant effect of Xixin Decoction and volatile oil mixture on reserpine-induced depression in rats and explore its mechanism.Methods：Except the normal control group，intraperitoneal injections of reserpine was given 0.3 mg/kg abdominal cavity injection every day.The rats in each group were given orally for 12 consecutive days after the 4th day of intraperitoneal injection.The changes of body weight and rectal temperature of rats，the distance of movement and the number of times of standing were observed.The contents of monoamine transmitters and their metabolites were determined by enzyme-linked immunosorbent assay （ELISA）.Results：Compared with the control group，body weight，the rectal temperature，the distance of behavioral movement and standing times in the model group were significantly reduced （P<0.01），the contents of serotonin （5-HT），dopamine （DA） and adenaline （NE） in the brain tissue of the model group were significantly decreased （P<0.01）.com

pared with the model group，the high，medium and low doses of Xixin decoction and volatile oil mixture can correct the decreasing trend of of body weight，rectal temperature，behavioral distance and standing times induced by reserpine to different degrees，and increase the content of 5-HT，DA and NE in the rat brain tissue，and decrease the content of 5-hyroxyindole acetic （5-HIAA），4-dihydroxyphenylacetic （DOPAC），homovanillic （HVA） in the rat brain tissue.Conclusion：Xixin Decoction and volatile oil mixture showed a good antidepressant effect on reserpine-induced depression in rats，and the antidepressant effect may be related to its ability to enhance the synthesis of monoamine transmitters and inhibit the metabolism of monoamine transmitters.Quality metabolism is closely related.

Keywords Herba Asari; Depression; Mechanism; Monoamine transmitters

中图分类号：R242;R285.5文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.01.013

抑郁症是一种复杂的心理障碍性疾病，其病情严重，发病率逐年增加、发病人群广[1-3]。当前治疗抑郁症多采用的是西药，主要有盐酸氟西汀、盐酸文拉法辛、丙咪嗪等代表性药物[4]。上述药物对抑郁症有一定的治疗作用，但在使用过程中也出现了非常多的不良反应。中医药虽没有抑郁症病名，但通过辨证施治有较好的的疗效和较大的安全优势。因此，有必要从中药中发掘有效药物，为防治抑郁症服务。

细辛是马兜铃科细辛属植物北细辛Asarum heterotropoides Fr.Schmldt var.mandshuricum（Maxim）Kitag.、汉城细辛Asarum sieboldii Miq.Asarumsieboldii Miq.var.seoulense Nakai与华细辛Asarum sieboldii Miq.的干燥根和根茎，味辛、性温，具有祛风散寒、通窍止痛、温肺化饮的功效，用于风寒感冒、头痛、牙痛、鼻塞鼻渊、风湿痹痛、痰饮咳喘等[5-7]。细辛挥发油中含黄樟脑、3-蒈烯、3，5-二甲氧基甲苯、丁香酚甲醚苯等[8]。现代药理学研究表明细辛具有强心、解热镇痛、镇静、局部麻醉、抗炎等多种药理作用[9]。……