聂美楠

【摘要】 目的：探究CD34、podoplanin与子宫颈癌细胞增殖、血管内皮生长因子的关系及对预后的影响。方法：选取2015年5月-2017年5月本院子宫颈癌患者106例，均行手术切除治疗，留取子宫颈癌组织与癌旁正常组织标本。比较子宫颈癌组织与癌旁正常组织CD34、podoplanin表达情况，分析子宫颈癌组织中CD34与podoplanin的相关性，并对比不同CD34、podoplanin表达子宫颈癌组织增殖基因（Derlin1、SFRP2、TUG1、PRPS2）、血管内皮生长因子[血管内皮生长因子（VEGF）-A、VEGF-C、VEGF-D、血管内皮生长因子受体3（VEGFR-3）]水平，分析CD34、podoplanin与增殖基因、血管内皮生长因子的关系。随访3年，统计分析患者3年生存率。结果：子宫颈癌组织CD34、podoplanin阳性表达率、表达量均高于癌旁组织（P<0.05）。子宫颈癌组织中CD34表达量与podoplanin表达量存在正相关关系（P<0.05）。CD34强阳性子宫颈癌组织Derlin1、TUG1、PRPS2表达量高于弱阳性子宫颈癌组织，SFRP2低于弱阳性子宫颈癌组织（P<0.05）;podoplanin阳性子宫颈癌组织Derlin1、TUG1、PRPS2表达量高于阴性子宫颈癌组织，SFRP2低于阴性子宫颈癌组织（P<0.05）;CD34强阳性子宫颈癌组织VEGF-A、VEGF-C、VEGF-D、VEGFR-3表达量高于弱阳性子宫颈癌组织（P<0.05）;podoplanin阳性子宫颈癌组织VEGF-A、VEGF-C、VEGF-D、VEGFR-3表达量高于阴性子宫颈癌组织（P<0.05）。子宫颈癌组织CD34、podoplanin与Derlin1、TUG1、PRPS2、VEGF-A、VEGF-C、VEGF-D、VEGFR-3均存在正相关关系，与SFRP2存在负相关关系（P<0.05）。CD34强阳性患者3年生存率低于弱阳性者，podoplanin阳性患者3年生存率低于阴性者（P<0.05）。结论：子宫颈癌组织CD34、podoplanin表达水平与子宫颈癌细胞增殖基因表达、血管内皮生长因子间存在相关性，且CD34、podoplanin陽性表达可降低患者生存率，早期检测其表达情况有助于评定子宫颈癌预后。

【关键词】 子宫颈癌 CD34 podoplanin 细胞增殖 血管内皮生长因子 生存率

Relationship between CD34， podoplanin and Cervical Cancer Cell Proliferation， Vascular Endothelial Growth Factor and Their Influence On Prognosis/NIE Meinan. //Medical Innovation of China， 2021， 18（13）： 0-028

[Abstract] Objective： To explore the relationship between CD34， podoplanin and cervical cancer cell proliferation， vascular endothelial growth factor and their influence on prognosis. Method： A total of 106 cervical cancer patients in our hospital from May 2015 to May 2017 were selected， all of whom underwent surgical resection， and specimens of cervical cancer tissues and adjacent normal tissues were retained. The expression of CD34 and podoplanin in cervical cancer tissue and normal tissue adjacent to the cancer were compared， and the correlation between CD34 and podoplanin in cervical cancer tissue was analyzed. And the different CD34， podoplanin expression of cervical cancer tissue proliferation genes （Derlin1， SFRP2， TUG1， PRPS2）， vascular endothelial growth factor [vascular endothelial growth factor-A （VEGF-A）， VEGF-C， VEGF-D， vascular endothelial growth factor receptor 3 （VEGFR-3）] levels were compared. The relationship between CD34， podoplanin， proliferation genes and vascular endothelial growth factor was analyzed. Followed up for 3 years， the 3-year survival rate was statistically analyzed. Result： The positive expression rates and levels of CD34 and podoplanin in cervical cancer tissues were higher than those in adjacent tissues （P<0.05）. There was a positive correlation between the expression of CD34 and podoplanin in cervical cancer tissues （P<0.05）. The expression levels of Derlin1， TUG1 and PRPS2 in CD34 strongly positive cervical cancer tissues were higher than those in weakly positive cervical cancer tissues， and SFRP2 was lower than weakly positive cervical cancer tissues （P<0.05）; the expression levels of Derlin1， TUG1 and PRPS2 in podoplanin-positive cervical cancer tissue were higher than those in negative cervical cancer tissue， and SFRP2 was lower than that in negative cervical cancer tissue （P<0.05）; the expression levels of VEGF-A， VEGF-C， VEGF-D， and VEGFR-3 in CD34 strongly positive cervical cancer tissues were higher than those in weakly positive cervical cancer tissues （P<0.05）; the expression levels of VEGF-A， VEGF-C， VEGF-D and VEGFR-3 in podoplanin-positive cervical cancer tissues were higher than those in negative cervical cancer tissues （P<0.05）. CD34， podopranin and Derrin1， Tug1， PRPS2， VEGF-A， VEGF-C， VEGF-D and VEGFR-3 were positively correlated with cervical cancer tissues， and there was a negative correlation with SFRP2 （P<0.05）. The 3-year survival rate of CD34-positive patients was lower than that of weak-positive patients， and the 3-year survival rate of podoplanin-positive patients was lower than that of negative patients （P<0.05）. Conclusion： The expression levels of CD34 and podoplanin in cervical cancer tissues are correlated with the expression of proliferation genes and vascular endothelial growth factor in cervical cancer cells. Moreover， the positive expression of CD34 and podoplanin can reduce the survival rate of patients， and early detection of the expression of CD34 and podoplanin is helpful to evaluate the prognosis of cervical cancer.

[Key words] Cervical cancer CD34 podoplanin Cell proliferation Vascular endothelial growth factor

Survival rate

First-authors address： Hong Da Hospital of Jiamusi University， Jiamusi 154002， China

doi：10.3969/j.issn.1674-4985.2021.13.006

子宫颈癌是全球范围内女性第二大恶性肿瘤，每年可导致26万人死亡，我国当前子宫颈癌的发生率逐渐增高，且呈现年轻化趋势，早期确诊疾病、明确病情是当下该病治疗结局优化的关键环节[1-2]。转移和侵袭是恶性肿瘤的基本特征，子宫颈癌尤以淋巴转移为主，是导致患者死亡的主要原因[3]。近年来研究发现，podoplanin是一个特异性表达于淋巴管内皮的分子，被认为是淋巴管内皮特异性标记物，具有促进肿瘤淋巴管形成的作用，且与肿瘤预后密切相关[4]。CD34是一种血管标记物，其阳性表达可刺激肿瘤组织中脉管的增加，促进肿瘤的转移、浸润[5-6]。但目前关于CD34、podoplanin在子宫颈癌组织中表达情况的研究较少，其具体应用价值尚未明确。基于此，本研究尝试探究CD34、podoplanin与子宫颈癌细胞增殖、血管内皮生长因子的關系及对预后的影响，旨在探寻子宫颈癌靶向治疗的新途径。……