赵洪超 关书博 王丹

摘要 目的：探究不同炮制方法對黄柏药效的影响。方法：选择Balb/c小鼠为实验动物，将其随机分为蜜制组、盐制组、酒制组、生品组与模型组，每组10只，建立溃疡性结肠炎模型，蜜制组、盐制组、酒制组、生品组给予对应药液灌胃干预，比较各组疾病活动指数（DAI）、结肠长度、结肠大体形态损伤指数、组织学评分及结肠组织炎症介质[肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-17（IL-17）]、氧化应激指标[超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）]差异。结果：其中蜜制组、酒制组DAI及组织学评分均明显低于盐制组、生品组，结肠组织TNF-α、IL-17水平均明显低于盐制组、生品组，结肠组织IL-10水平均明显高于盐制组、生品组，差异有统计学意义（P<0.05）;蜜制组、盐制组、酒制组结肠长度及结肠组织SOD、GSH-Px水平均明显高于生品组，结肠大体形态损伤指数及结肠组织MDA水平均明显低于生品组，差异有统计学意义（P<0.05）。结论：不同方法炮制黄柏均可增强对溃疡性结肠炎的药效，尤以蜜制、酒制黄柏增强效果较为突出，可为其炮制工艺提供参考。

关键词 黄柏;炮制;药效;溃疡性结肠炎;小鼠;疾病活动指数;结肠长度;结肠大体形态损伤指数;组织学评分;炎症介质;氧化应激指标

Effects of Different Processing Methods on Ulcerative Colitis Mice Pharmaceutical Effects of Cortex Phellodendri

ZHAO Hongchao，GUAN Shubo，WANG Dan

（The Second Hospital of Traditional Chinese Medicine，Shenyang 110101，China）

Abstract Objective：To explore the effects of different processing methods on pharmaceutical effects of Cortex Phellodendri.Methods：Balb/c mice were selected as experimental animals.And were randomly divided into a ulcerative colitis group，a honey processing group，a salt processing group，a wine processing group，a crude herb group respectively，with 10 mice in each group.The disease activity index （DAI），colon length，colon gross morphological damage index，histological score and colonic cytokines [tumor necrosis factor-α （TNF-α），interleukin-10 （IL-10），interleukin-17 （IL-17）] and oxidative stress indicators [superoxide dismutase （SOD），malondialdehyde （MDA），glutathione peroxidase （GSH-px）] were compared between the 2 groups.Results：The DAI and histological score in honey processing group and wine processing group were significantly lower than those in salt processing group and crude herb group.The levels of TNF-α and IL-17 in colon tissues were significantly lower than those in salt processing group and crude herb group while the level of IL-10 in colon tissues was significantly higher than that in salt processing group and crude herb group （P<0.05）.The colon length and levels of SOD and GSH-px in colon tissues in honey processing group，salt processing group and wine processing group were significantly higher than those in crude herb group，and the colon gross morphological damage index and the MDA level in colon tissues were significantly lower than those in crude herb group （P<0.05）.Conclusion：Different processing methods of Cortex Phellodendri can enhance the pharmaceutical effects of ulcerative colitis，especially the honey-processing and wine-processing Cortex Phellodendri have more prominent enhancement effect，which can provide reference for processing technology.

Keywords Cortex phellodendri; Processing; Pharmaceutical effects; Ulcerative colitis; Mice; Disease activity index; Colon length; Colon gross morphological damage index; Histological score; Cytokines; Oxidative stress indicators

中图分类号：R282;R574文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.04.015

黄柏是芸香科植物黄皮树的干燥树皮，常言道“檗木，黄檗也，今处处有之，以蜀中者为佳”，该药材性寒味苦，归肾、膀胱经，有清热燥湿、泻火除蒸、解毒疗疮之功效，主治以湿热泻痢、热淋涩痛、疮疡肿毒、热淋涩痛等疾病为代表的热证[1]。传统的黄柏炮制方法较多，《证治准绳》记载“生用降实火，熟用酒制治上，盐制治下，蜜制治中不伤胃”[2]，可见古代医家对黄柏炮制及辨证用药的经验总结已较为全面，但未能从药代动力学、药效学的角度获得解释。目前黄柏复方或组方治疗溃疡性结肠炎的效果已获得广泛证实[3-7]，但黄柏具体如何影响治疗效果仍不十分明确。……