孙晶琦 许炜清 孙晨 许晓倩 陈晶晶 王晓立

摘 要：WRKY转录因子是植物中最大的转录调控家族之一，是调控植物许多生物过程信号网络的组成部分，具有多种生物学功能。该研究根据已公布的拟南芥基因组中当前确定的WRKY基因家族成员，利用其序列进行结构域的分析，从而确定WRKY基因家族的基本特征，并以此为基础，从毛果杨序列库中筛选出WRKY基因家族的成员。再利用MEGA5软件、在线工具ExpasyProtparma、BioEdit等进行理化性质分析、motif结构对比、进化树构建、基因表达分析等。结果表明：毛果杨WRKY基因家族20个成员编码序列长为189～624bp，有多个染色体定位重复分布于第1、5、14号染色体上。WRKY蛋白均定位于细胞外，亲水性较差，具有较高的脂溶性和不稳定性。20个WRKY基因均在毛果杨中表达，但有明显的差异。

关键词：毛果杨;WRKY蛋白;基因家族;生物信息

中图分类号 Q943.2文献标识码 A文章编号 1007-7731（2021）09-0016-04

Abstract： WRKY transcription factors， one of the largest transcriptional regulatory families in plants， are part of the signal networks that regulate many biological processes in plants， and have a variety of biological functions. In this study， the WRKY gene family members were identified in Arabidopsis thaliana on the published Arabidopsis genome， and the basic characteristics of the WRKY gene family were determined by using their sequences for domain analysis. On this basis， the members of the WRKY gene family were screened out from the sequence library of Populustrichocarpa. MEGA5 software， online tools such as ExPASyProtparma and Bioedit were used to analyze physical and chemical properties， motif structure comparison， evolutionary tree construction and gene expression analysis. The results showed that the coding sequence length of 20 members of the WRKY gene family was 189～624bp， and multiple chromosomes were located and repeated on chromosomes 1， 5 and 14. All WRKY proteins were extracellular， with poor hydrophilicity and high lipid solubility and instability. All the 20 WRKY genes were expressed in Populustrichocarpa， but there were significant differences.

Key words： Populustrichocarpa; WRKY proteins; Gene family; Biological information

轉录调控是生物在生长发育及代谢过程中调控细胞或组织中基因表达的重要机制[1]。转录因子（Transcription factors，TFs）是一类锚定在目标基因启动子区域特异序列上的蛋白质，能对下游目标基因的转录进行促进或抑制作用，对生物体的正常生长发育和应对外界胁迫过程都具有重要的作用[1，2]。WRKY基因家族是近年来发现的一类植物特有的转录调节因子，它是一种植物特有的调控因子，已从多种植物中分离，如甜土豆[3]、野燕麦[4]、皱叶欧芹[5]、拟南芥[6]、烟草[7]等。根据目前所有的研究资料来看，WRKY蛋白在植物的各项生理活动过程中发挥着重要的调控作用，如胚胎形态的发生[8]、叶片的衰老等[9]，同时在非生物胁迫包括寒冷、干旱、盐碱等[10]和生物胁迫包括细菌、真菌、病毒等[11]逆境响应及信号转导过程中也起着重要的调控作用。……