黎飞彤 方高根 王彩冰 孙悦 黄章南 黎智丹 王秋君

【摘要】 目的 探討白花九里明对高血压大鼠模型的心血管功能保护作用。

方法 将SD大鼠48只随机均分为正常对照组和高血压模型组、替米沙坦组及白花九里明低、中、高浓度组，每组8只大鼠，每天灌胃一次，灌胃量按大鼠体重10 mL/kg计算，连续7天;同时，正常对照组给予0.9%氯化钠注射液，其余各组给予5 mg/mL地塞米松注射液，每天皮下注射一次，注射量按大鼠体重3 mL/kg计算，连续7天。各组大鼠于末次用药后30 min经左侧颈总动脉插管和右侧颈外静脉插管测定收缩压（SBP）、舒张压（DBP）、心率（HR）、中心静脉压（CVP）。

结果 高血压模型组的SBP、DBP明显高于其余各组（P<0.05或0.01），替米沙坦组的SBP、DBP明显低于其余各组（P<0.05或0.01）。白花九里明低、中、高浓度组和正常对照组的SBP、DBP在组间两两比较差异均无统计学意义（P>0.05）。各组的HR、CVP在组间两两比较均无统计学意义（P>0.05）。

结论 白花九里明能抵消地塞米松诱导高血压模型的血管收缩和高血压现象，使血压恢复正常，也能消除地塞米松对心脏生物电干扰和心泵功能降低的不良趋向，达到保护心血管功能的作用。

【关键词】 白花九里明;高血压模型;血压;心率;中心静脉压;大鼠

中图分类号：R29;R932;R544.1;R285.5   文献标志码：A   DOI：10.3969/j.issn.1003-1383.2021.04.003

Experimental study on protective effect of Zhuang medicine blumea megacephala on cardiovascular function in rat model of hypertension

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LI Feitong1， FANG Gaogen1， WANG Caibing2， SUN Yue3， HUANG Zhangnan3， LI Zhidan3， WANG Qiujun3

（1.Undergraduate of Clinical Medicine， Grade 2016， 2. Department of Physiology， 3. Undergraduate of Clinical Medicine， Grade 2017， Youjiang Medical University for Nationalities， Baise 533000， Guangxi， China）

[HJ2][HJ]

【Abstract】 Objective To explore the protective effect of blumea megacephala on cardiovascular function in hypertensive rat model.

Methods 48 SD rats were randomly divided into normal control group and hypertension model group， telmisartan group， blumea megacephala low， medium and high concentration groups， with 8 cases in each group. The rats were intragastrically administered once a day for 7 consecutive days according to body weight （10 mL/kg）. At the same time， the normal control group were given 0.9% sodium chloride injection， and the other groups were given 5 mg/mL dexamethasone injection， subcutaneous injection was performed once a day for 7 consecutive days， the injection volume was calculated according to the weight of rats （3 mL/kg）. 30 minutes after the last administration， systolic blood pressure （SBP）， diastolic blood pressure （DBP）， heart rate （HR） and central venous pressure （CVP） of each group were measured by left common carotid artery intubation and right vena jugularis externa intubation.

Results SBP and DBP in the hypertension model group were significantly higher than those in other groups （P < 0.05 or 0.01）， SBP and DBP in the telmisartan group were significantly lower than those in other groups （P < 0.05 or 0.01）. There was no statistically significant difference in SBP and DBP between the blumea megacephala low， medium and high concentration groups and the normal control group （P > 0.05）. Difference of HR and CVP in pairwise comparison between groups was not statistically significant （P > 0.05）.

Conclusion Blumea megacephala can not only counteract the vasoconstriction and hypertension phenomenon of dexamethasone induced hypertension model， make the blood pressure return to normal， but also eliminate the adverse trend of dexamethasone on cardiac bioelectricity interference and cardiac pump function reduction， so as to protect cardiovascular function.

【Key words】 blumea megacephala; hypertensive model; blood pressure; HR; CVP; rat

白花九里明（blumea megacephala 〔Randeria〕 Chang et Tseng）属菊科植物，在壮族民间常用于治疗跌打肿痛、风湿骨痛、产后出血、月经不调等[1～2]。近年有文献报道[3]，白花九里明具有镇痛抗炎、保肝护心、促凝止血、降压升糖和降低心肌收缩力等作用。因此，本研究以白花九里明民间用量[1～2]按体表面积换算成大鼠用量给大鼠灌胃，研究白花九里明对地塞米松（dexamethasone，DM）诱导高血压大鼠模型[4]的血压、心率和中心静脉压的影响，并与降压药替米沙坦的临床用量[5]按体表面积换算成大鼠用量给大鼠灌胃作比较分析，探讨白花九里明对心血管功能保护作用及机制，为白花九里明开发和利用提供依据。