蚕豆遗传图谱与QTL定位研究进展
2021-03-25周仙莉滕长才张红岩林夕刘玉玲吴小燕侯万伟刘玉皎
周仙莉 滕长才 张红岩 林夕 刘玉玲 吴小燕 侯万伟 刘玉皎


摘要: 作为一种粮食、蔬菜、饲料、绿肥兼用的食用豆类,蚕豆在人类健康、土壤改良等方面发挥了重要作用。高密度遗传图谱的构建是蚕豆基因组研究的基础和主要手段,至今共构建了24张蚕豆遗传图谱,最为饱和的图谱覆盖长度为1 439 cM,平均遗传距离为0.8 cM。同时基于图谱开展了产量相关性状、品质相关性状以及抗性相关性状的QTL定位,有效地促进了蚕豆的遗传与基因研究。
关键词: 蚕豆;遗传连锁图谱;QTL定位
中图分类号: S529 文献标识码: A 文章编号: 1000-4440(2021)01-0237-09
Research advance of genetic linkage map and QTL location in Vicia faba L.
ZHOU Xian-li1,2, TENG Chang-cai2, ZHANG Hong-yan1,2, LIN Xi1,2, LIU Yu-ling1,2, WU Xiao-yan1,2,HOU Wan-wei2, LIU Yu-jiao1,2,3
(1.Qinghai University, Xining 810016, China;2.Qinghai Academy of Agricultural and Forestry Sciences, Xining 810016, China;3.The Co-constructing State Key Laboratory of Three Rivers Sources Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
Abstract: As a kind of edible legumes which can be used as food, vegetables feed and green manure, Vicia faba L. plays a crucial role in human health and soil improvement. The construction of high-density genetic linkage map is the basis and main method for genome research in V. faba L.. 24 genetic maps have been constructed so far, in which the most saturated map spanned 1 439 cM with an average genetic distance of 0.8 cM. Quantitative trait locus (QTL) mapping on yield-related traits, quality-related traits, and resistance-related traits had been carried out, which effectively promoted inheritance and gene research in V. faba L..
Key words: Vicia faba L.;genetic linkage maps;quantitative trait locus (QTL) location
蠶豆(Vicia faba L.)具有“养人、养畜、养地”的作用,是中国传统的出口农产品,也是青海省种植业结构调整和农民增收的优势作物之一[1-3]。随着人们生活水平的提高和健康饮食需求的增加,菜用蚕豆的市场需求量越来越大。蚕豆作为一种轮作豆类,在土壤改良方面也发挥着重要作用。
遗传连锁图谱是指已知基因或分子标记在连锁群上的线性排列,标记间的相对遗传距离通过重组率计算[4-5]。蚕豆基因组庞大(约13 Gb),缺乏可用的基因组序列数据,导致其基因组研究进展缓慢[6]。构建高密度遗传图谱,进行QTL定位以及开发连锁标记是蚕豆基因组研究的主要手段,对控制重要性状的关键基因定位与克隆、关联与连锁分析以及分子标记辅助选择优良品种具有重要作用。
基于蚕豆遗传图谱,对产量、品质、抗性等重要性状进行基因与QTL定位,并针对部分性状开发连锁标记,有利于替代繁琐且耗时的表型评估,促进分子标记辅助选择(MAS)育种的发展,进而缩短育种进程[7]。……
