高阳　侯非凡　熊雄　公菲菲　王金耀　邢国明　李森

摘要： 为了更加精准地开展黄花菜基因组组装和性状定位，在课题组前期构建的大同黄花×摇篮曲种间遗传图谱的基础上，基于大同黄花全基因组和转录组数据库，针对秋水仙碱及昼夜节律2个关键性状所涉及的候选Unigene开发基因组SSR标记，进一步通过55个F1后代加密图谱。结果表明，新开发的95个gSSR标记中有46个标记在亲本及子代间具有多态性，多态性比例为48.42%，有22个gSSR标记到图谱上，同时有12个前期未上图的EST-SSR标记加密到新图谱。加密后的遗传图谱包含255个标记，由9个连锁群组成，总图距为3 142.64 cM，平均图距为11.88 cM，各连锁群标记数量为5～58个。研究结果为进一步研究黄花菜秋水仙碱含量及昼夜节律的功能基因定位奠定了重要基础。

关键词： 黄花菜;gSSR;标记开发;图谱加密

中图分类号： Q755 文献标识码： A 文章编号： 1000-4440（2021）01-0139-11

Enhancement of the interspecific genetic map density for Hemerocallis citrina and H. fulva by developing gSSR markers based on candidate genes

GAO Yang1，2， HOU Fei-fan1，2，3， XIONG Xiong1，2， GONG Fei-fei1，2， WANG Jin-yao1，2，3， XING Guo-ming1，2，3， LI Sen1，2，3

（1.College of Horticulture，Shanxi Agricultural University，Taigu 030801，China;2.Datong Huanghua Industry Development Research Institute，Datong 037004，China;3.Collaborative Innovation Center of Quality and Profit Improvement for the Facility Vegetables of Shanxi Province，Taigu 030801，China）

Abstract： To carry out genome assembly and character mapping of Hemerocallis citrina more accurately， genomic simple sequence repeats （SSR） markers were developed for the candidate Unigenes involved in two key characters （colchicine and circadian rhythm）， on the basis of the interspecific genetic map of DatongHuanghua×Lullaby Baby constructed by our research group previously and the whole genome and transcriptome database of DatongHuanghua. The map density was further enhanced through 55 F1 progenies. The results showed that 46 of the 95 newly developed gSSR markers were polymorphic between parents and offsprings， with a polymorphic ratio of 48.42%. 22 gSSR markers were added to the map， while 12 EST-SSR markers not mapped earlier were added to the new map. The updated genetic map contained 255 markers which was composed of nine linkage groups. The total map distance was 3 142.64 cM， and the average map distance was 11.88 cM. The number of markers in each linkage group ranged from 5 to 58. The results can make important foundation for further study on colchicine content and functional gene mapping of circadian rhythm in H.citrina.

Key words： Hemerocallis citrina;gSSR;marker development;density enhancement of the genetic linkage map

萱草屬（Hemerocallis spp.）植物是世界著名的宿根花卉之一，Li等[1]将155个萱草属植物品种分为黄花菜（Hemerocallis citrina）等夜间开花类群和萱草（Hemerocallis fulva）等白天开花类群。黄花菜花蕾营养价值丰富，又名“金针菜”，是一种重要的药食兼用型功能蔬菜[2-4]，产量、品质和营养成分含量是其重要的育种目标，黄花菜中富含多种次生代谢物，这些物质在现代医学及生物学研究中发挥着重要作用[5]。黄花菜新鲜花蕾中含有次生代谢物秋水仙碱[6-7]，有报道显示鲜食黄花菜可能产生中毒现象，导致腹泻等不良反应[8]。萱草花色、花型丰富，耐干旱瘠薄，是重要的园林绿化材料，其育种主要围绕改良观赏性状、提升观赏价值来进行[9]，萱草的观赏性主要体现在花器官，而萱草属植物花器官存在明显的昼夜节律现象，该现象成为影响其观赏性的重要因素。……