张帅

【摘要】 目的：探究外周血T、B淋巴细胞亚群及趋化因子在慢性荨麻疹患者中的检测价值。方法：选取2019年3月-2021年1月本院93例慢性荨麻疹患者为观察组，选取同期93例体检健康者为对照组。比较两组的外周血T淋巴细胞亚群（CD3+、CD8+、CD4+/CD8+）、B淋巴细胞亚群（CD3-CD19+、CD19+）及趋化因子（CCL-27、MCP-1、Rantes）水平;比较观察组中不同荨麻疹活动性评分（UAS）者的外周血T、B淋巴细胞亚群及趋化因子水平;采用Spearman秩相关分析外周血T、B淋巴细胞亚群及趋化因子水平与UAS的关系。结果：观察组CD8+、CCL-27、MCP-1、Rantes均高于对照组，CD3+、CD4+/CD8+、CD3-CD19+、CD19+均低于对照组，差异均有统计学意义（P<0.05）。观察组中不同UAS者的外周血T、B淋巴细胞亚群和血清趋化因子比较，差异均有统计学意义（P<0.05）。UAS>4分患者的CD8+、CCL-27、MCP-1、Rantes均高于UAS为0～2分和3～4分患者，CD3+、CD4+/CD8+、CD3-CD19+、CD19+均低于UAS為0～2分和3～4分患者，差异均有统计学意义（P<0.05）。UAS为3～4分患者的CD8+、CCL-27、MCP-1、Rantes均高于UAS为0～2分患者，CD3+、CD4+/CD8+、CD3-CD19+、CD19+均低于UAS为0～2分患者，差异均有统计学意义（P<0.05）。Spearman秩相关分析显示，CD8+、CCL-27、MCP-1、Rantes与UAS均呈正相关，其CD3+、CD4+/CD8+、CD3-CD19+、CD19+与UAS均呈负相关（P<0.05）。结论：外周血T、B淋巴细胞亚群及趋化因子在慢性荨麻疹患者中异常表达，且与疾病活动度有密切的关系，因此在慢性荨麻疹患者中的检测价值较高。

【关键词】 T淋巴细胞亚群 B淋巴细胞亚群 趋化因子 慢性荨麻疹

Investigation on the Detection Value of Peripheral Blood T and B Lymphocyte Subsets， Seurm Chemokines in Patients with Chronic Urticaria/ZHANG Shuai. //Medical Innovation of China， 2021， 18（32）： 0-067

[Abstract] Objective： To investigate the detection value of peripheral blood T and B lymphocyte subsets， seurm chemokines in patients with chronic urticaria. Method： A total of 93 patients with chronic urticaria from March 2019 to January 2021 in our hospital were selected as the observation group， and 93 healthy subjects during the same period were selected as the control group. Peripheral blood T lymphocyte subsets （CD3+， CD8+， CD4+/CD8+）， B lymphocyte subsets （CD3-CD19+， CD19+） and seurm chemokines （CCL-27， MCP-1， Rantes） were compared between two groups. The levels of peripheral blood T and B lymphocyte subsets and serum chemokines in peripheral blood of the observation group with different urticaria activity score （UAS） were compared. The relationship between T and B lymphocyte subsets and chemokine levels in peripheral blood and UAS were analyzed by Spearman method. Result： CD8+， CCL-27， MCP-1 and Rantes in the observation group were higher than those in the control group， while CD3+， CD4+/CD8+， CD3-CD19+ and CD19+ in the observation group were lower than those in the control group， the differences were statistically significant （P<0.05）. There were statistically significant differences in peripheral blood T and B lymphocyte subsets and serum chemokines of patients with different UAS in the observation group （P<0.05）. CD8+， CCL-27， MCP-1 and Rantes in patients with UAS >4 were higher than those in patients with UAS of 0-2 and 3-4， and CD3+， CD4+/CD8+， CD3-CD19+ and CD19+ in patients with UAS >4 were lower than those in patients with UAS score 0-2 and 3-4， the differences were statistically significant （P<0.05）. CD8+， CCL-27， MCP-1 and Rantes in patients with UAS score of 3-4 were higher than those in patients with UAS score of 0-2， and CD3+， CD4+/CD8+， CD3-CD19+ and CD19+ in patients with UAS score of 3-4 were lower than those in patients with UAS score of 0-2， the differences were statistically significant （P<0.05）. Spearman correlation analysis showed that CD8+， CCL-27， MCP-1 and Rantes were positively correlated with UAS， while CD3+， CD4+/CD8+， CD3-CD19+， CD19+ were negatively correlated with UAS （P<0.05）. Conclusion： The peripheral blood T and B lymphocyte subsets， seurm chemokines in patients with chronic urticaria show abnormal expression state， and they are close with disease activity， so their detection value in the patients with chronic urticaria are higher.

[Key words] T lymphocyte subsets B lymphocyte subsets Seurm chemokines Chronic urticaria

First-author’s address： Anshan Central Hospital， Anshan 114000， China

doi：10.3969/j.issn.1674-4985.2021.32.016

慢性荨麻疹在临床具有较高的发病率，本病可导致患者出现风团、红斑及瘙痒等情况，严重影响患者的生存质量。临床中与慢性荨麻疹相关的研究显示，机体免疫是研究较热的方面[1-2]。有研究显示，本类患者处于免疫失衡的状态，而外周血T、B淋巴细胞亚群作为机体免疫的重要组成部分，其在慢性荨麻疹中的研究虽可见，但是研究结果差异显著[2-4]。……