吴谷丰　龚顺民　刘丽君　王凯旋　李青连　郝澍

摘 要：目的：探讨富硒酵母干预对高脂大鼠内质网应激基因GRP78表达的影响。方法：选取40只6周龄小鼠作为研究对象，建立高脂小鼠模型，并随机分为4组（a：对照组，b：高脂日粮组，c：高脂日粮+低剂量富硒酵母组，d：高脂日粮+高剂量富硒酵母组），观察肝脏脂肪变性程度，检测肝脏脂质氧化MDA水平和GRP78基因的mRNA表达水平。结果：高脂日粮可以诱发小鼠肝脏指数增大和脂肪变性，饲喂富硒酵母后，小鼠的肥胖和肝脏脂肪变性得到了明显改善;高脂日粮组与其他组相比肝脏中MDA和GRP78基因的表达水平显著升高，高剂量硒酵母对高脂诱发的MDA升高和GRP78基因的表达水平均有拮抗作用。结论：硒能逆转高脂小鼠的肝脏损伤，内质网应激信号通路在其中发挥了作用，该应激可能是通过提高氧化水平而诱导的。

关键词：高脂;富硒酵母;内质网应激;GRP78基因

中图分类号 R332文献标识码 A文章编号 1007-7731（2021）03-0012-04

Effect of Selenium Enriched Yeast Intervention on GRP78 Gene Expression in High-fat Mice

WU Gufeng1 et al.

（1School of Pharmacy and Life Science，Jiujiang University，Jiujiang 332000，China）

Abstract： Objective：To investigate the effect of selenium enriched yeast intervention on the endoplasmic reticulum stress gene GRP78 expression in high-fat rats. Methods：A total of 40 mice were selected as the research object to establish high-fat mouse model， and randomly divided into 4 groups （a： control group， b： high-fat diet group， c： high-fat diet + low dose of selenium enriched yeast group， d： high-fat diet + high dose group of selenium enriched yeast）. Then， the degrees of liver steatosis were observed， and the liver MDA level and lipid oxidation of GRP78 gene mRNA expression level were detected. Results：High-fat diet could induce the increase of liver index and alleviate steatosis in mice. Compared with other groups， the expression levels of MDA and GRP78 genes in liver were significantly increased， and the high-dose selenium enriched yeast had an inhibitive effect on increase of MDA and the expression levels of GRP78 genes. Conclusion：Selenium can reverse liver injury in high-fat mice， and endoplasmic reticulum stress signaling pathway plays a role in it， which may be induced by increased oxidation levels.

Key words： High-fat; Selenium enriched yeast; Endoplasmic reticulum stress; GRP78 gene

近些年来，随着人们生活水平的提高，心血管疾病的发病率呈逐年上升趋势，并已成为严重威胁着我国居民健康的重大疾病[1]。目前，诱发心血管疾病的病因较多且较复杂，其中高脂饮食引发的血脂异常是导致心血管疾病发生和发展的主要危险因素之一[2，3]。据國家心血管病中心统计显示，截至2018年，我国血脂异常人数已超过4亿人。研究表明，高脂饮食可诱导组织与细胞内产生并累积大量的自由基，从而引起氧化应激，对组织和细胞造成损伤，除此之外还能引起炎性反应和胰岛素抵抗[4，5]。……