陈美莲　章慧　丰东升　张维谊　韩奕奕　王敏　周雨璊　马颖清　汪弘康　王艺娇

摘要 [目的]研究膠体金法在蔬菜中多菌灵、吡虫啉和啶虫脒残留量检测的应用，并将该方法与液相色谱-串联质谱法比较，证实胶体金试纸条在检测蔬菜中多菌灵、吡虫啉和啶虫脒残留的适用性。[方法]试验验证了胶体金法检测蔬菜中多菌灵、吡虫啉和啶虫脒在不同基质中的灵敏度，并将该方法与液相色谱-串联质谱法比较验证其假阴性和假阳性率。[结果]胶体金法检测蔬菜中多菌灵、吡虫啉和啶虫脒的检出限分别为0.5、0.5和0.2  mg/kg;该方法在不同基质中的稳定性较好;实验室加标验证3种农药残留检测的假阴性率和假阳性率均为0，与参比方法无显著性差异（χ2<3.84）;实物阳性样品验证3种农药残留检测的假阴性率均为0，假阳性率分别为0、0和6.67%，但与参比方法均无显著性差异（χ2<3.84）。除多菌灵与阿苯达唑有100%交叉反应外，多菌灵、吡虫啉和啶虫脒与该研究中试验的常见农药均无交叉反应。[结论]胶体金法快速检测蔬菜中多菌灵、吡虫啉和啶虫脒操作简便、快速、准确、稳定，可作为部分蔬菜上市前残留检测的一种有效手段。

关键词 胶体金法;蔬菜;多菌灵;吡虫啉;啶虫脒;残留量

中图分类号 TS207.5+3文献标识码 A

文章编号 0517-6611（2020）22-0193-05

doi：10.3969/j.issn.0517-6611.2020.22.052

Study on Colloidal Gold Rapid Detecting Carbendazol，Imidacloprid and Acetamiprid in Vegetables

CHEN Mei-lian，ZHANG Hui，FENG Dong-sheng et al （Shanghai Agri-products Quality and Safety Center，The Ministry of Agriculture Food Quality Supervision and Inspection Center（Shanghai），Shanghai 201708）

Abstract [Objective]To study the application of colloidal gold method on detecting carbendazol，imidacloprid and acetamiprid in vegetables and compare to liquid chromatography-tandem mass spectrometry method to make sure the reliability of colloidal gold strip for determination of carbendazol，imidacloprid and acetamiprid residues in vegetables.[Method]The experiment verified the sensitivity of colloidal gold method for detecting carbendazim，imidacloprid and acetamiprid in vegetables in different matrices，and compared the method with liquid chromatography-tandem mass spectrometry to verify its false negative and false positive rates.[Result]The detection limit of colloidal gold rapid detecting carbendazol，imidacloprid and acetamiprid were 0.5 mg/kg，0.5 mg/kg and 0.2  mg/kg，their stabilities in different matrix was good.Laboratory labeling verification result showed that the false negative and positive rate were 0，no significant difference with reference method （χ2<3.84）.Physical positive sample verification result showed that their false negative were 0，their positive rate were 0，0 and 6.67%，no significant difference with reference method（χ2<3.84）.There were no cross-reaction between carbendazol，imidacloprid，acetamiprid and other pesticides except for 100% cross-reaction between carbendazol and albendazole.[Conclusion]Colloidal gold rapid detection of carbendazol，imidacloprid and acetamiprid in vegetables are simple，rapid，accurate and stable.It can be used as an effective method to detect the residues of carbendazol，imidacloprid and acetamiprid in vegetables.

Key words Colloidal gold method;Vegetables;Carbendazol;Imidacloprid;Acetamiprid;Residue

基金项目

上海市科技兴农重点攻关项目（2019-02-08-00-15-F01147）。

作者简介 陈美莲（1963—），女，上海人，农业推广研究员，从事农产品质量安全检测及风险评估工作。

收稿日期 2020-04-02;修回日期 2020-05-11

我国是一个农业大国，农药使用品种多、用量大，蔬菜中的农药残留严重影响了我国食用农产品的质量信誉，更损害了消费者的身体健康。目前，专属性强、灵敏度高的农药残留检测方法均为检测费用高、周期长、操作复杂的仪器分析方法。为提高监管与检验效率，降低监管部门、检验部门、种植基地、商家和其他市场流通环节对重点、高风险农药残留项目的监测的成本，解决上述監管需求，急需开发具有特异性强、灵敏度高、样品前处理简单的农药残留快速检测方法。……