杨兴变 郑雯 何珺 康冀川 李洪庆

摘 要：本文建立了一种利用大孔树脂分离富集缺陷假单胞菌HD13发酵液中抑制水稻纹枯病菌活性物质的方法。以水稻纹枯病菌为测試病原菌，采用缺陷假单胞菌HD13发酵液考察D101、LX-60、XDA-6、LSA-12、XDA-8G、LX-17共

6种大孔树脂对HD13菌株发酵液中抑制水稻纹枯病菌活性物质的分离效果。结果表明：XDA-8G树脂对其有很好的分离和富集作用，主要集中在40%乙醇洗脱液中，该方法成本低、操作简单、快速，可为后续大量发酵提供实践依据。

关键词：缺陷假单胞菌发酵液;活性物质;大孔树脂;水稻纹枯病菌

中图分类号：TQ920.6

文献标识码：A

文章编号：1008-0457（2020）02-0085-04 国际DOI编码：10.15958/j.cnki.sdnyswxb.2020.03.015

Study on the method for enriching and isolating the inhibiting active substances of Thanatephorus cucumeris from fermantientation broth of Pseudomonasdiminita HD13 by Macro-Porous Adsorption Resin

YANG Xingbian.1， ZHEN Wen.1，2，HE Jun.1，KANG Jichuan.1*，LI Hongqing.3*

（1.Engineering and Research Center of Guizhou Province of China，Guizhou University，Guiyang，Guizhou 550025，China ; 2.College of Pharmacy，Guizhou University，Guiyang，Guizhou 550025，China;3. College of Chemistry and Environmental Science，Guizhou Minzu University，Guiyang，Guizhou 550025，China ）

Abstract：In this paper， a method for separating and enriching inhibiting active substances of Thanatephorus cucumeris from the fermentation broth of Pseudomonasdiminita HD13 by using macroporous resin was established. Using Thanatephorus cucumeris as the test pathogen， six macroporous resins （D101， LX-60， XDA-6， LSA-12， XDA-8G and LX-17） were used to separate the inhibiting active substances of Thanatephorus cucumeris from the fermentation broth of Pseudomonasdiminita HD13.The results showed that XDA-8G resin had a good separation and enrichment effect. The inhibiting active substances were concentrated in the 40% alcohol eluent. The method has low cost， simple and fast operation， and can provide practical basis for subsequent large-scale fermentation.

Keywords：the fermentation broth of Pseudomonasdiminita; active substances; macroporous resin;Thanatephorus cucumeris

缺陷假单胞菌属于假单胞杆菌科，假单胞杆菌属，球菌类型，是一种常见的革兰氏阴性好氧杆菌，能够利用多种有机物，为自身提供碳源和能源。因而在自然界中分布十分广泛，其专性需氧，可在植物根系内部建立种群并抑制其它微生物的生长，其产生的 2，4-二乙酰滕黄酚、藤黄绿菌素、吩嗪类、脂多肽、硝吡咯菌素、氢氰酸等类型的次级代谢产物对多种微生物具有抑制作用[1-3]。BURR等[4]报道荧光假单胞菌对甜菜、马铃薯等作物具有增产作用;WELLER等[5]

利用荧光假单胞菌防治小麦全蚀病;THOMASSHOW等[6]利用恶臭假单胞菌抑制镰刀菌厚垣孢子的萌发;杨艺炜[7]发现绿针假单胞菌对烟草黑胫病及其游动孢子有抑制作用。此外，假单胞菌属对棉花黄萎病、水稻稻瘟病、水稻鞘腐病、烟草黑胫病、小麦全蚀病、番茄斑萎病毒等均有较好的防治效果[8-11]。……