马家玉　罗雯　艾佐佐　李丽群　林丽珍

摘要 [目的]以宁都黄鸡为试验对象，分析宁都黄鸡血清营养物质含量和血清免疫活性物质及盲肠消化酶活性与体重的相关性。[方法]选取健康状态良好的雄性宁都黄鸡150羽，散养条件下饲养至72日龄，按照体重由高到低分为较重组、中等组和较轻组。每组随机抽取样本10只，当日屠宰取血、静置并离心取血清，解剖后无菌取盲肠段内容物，以上样品置于-70 ℃冰箱中保存。血清总蛋白含量采用双缩脲常规法检测;血清白蛋白含量采用溴甲酚绿法检测;血清葡萄糖含量采用邻甲苯胺法检测，血清总胆固醇和甘油三酯含量采用GPO-PAP法测定;血清超氧化物歧化酶活性采用羟胺法测定;过氧化氢酶活性采用钼酸铵法测定;血清谷胱甘肽过氧化物酶和盲肠脂肪酶、胰蛋白酶活性均采用比色法测定;盲肠α淀粉酶活性采用淀粉-碘显色法测定。[结果]各体重组甘油三酯含量以及过氧化氢酶、总超氧化物歧化酶、胰蛋白酶和α淀粉酶活性均无显著差异，各生化指标与体重无相关性;各体重组总胆固醇含量和脂肪酶活性无显著差异，但与体重分别呈极显著正相关（P<0.01）和显著负相关（P<0.05）;各体重组谷胱甘肽过氧化物酶活性存在极显著差异（P<0.01），且与体重呈极显著负相关。[结论]该研究结果对宁都黄鸡饲料中营养的合理配比及外源性消化酶的适时添加具有一定的指导作用。

关键词 宁都黄鸡;体重;免疫活性物质;消化酶;相关性

中圖分类号 S831.8+9;S831.4文献标识码 A文章编号 0517-6611（2020）11-0116-03

Abstract [Objective] Ningdu Huang chicken were selected as experimental objects to analyze the correlation between serum nutrient content， immunologically active substances， cecal digestive enzyme activity and body weight of Ningdu Huang chicken. [Method] 150 male Ningdu Huang chicken in good health were raised to the age of 72 dayold under freerange conditions. According to the difference in body weight， they were divided into three groups from the highest to the lowest： the heavy group， the medium group and the light group. 10 samples were randomly selected from each group， respectively. On the day of slaughter， blood samples were taken and the serum was isolated after standing and centrifugation. After dissecting， the contents of cecum segment were extracted and stored in refrigerator at -70 ℃. Subsequently， the total serum protein content was detected by biuret method. The serum albumin content was detected by bromocresol green method. Serum glucose content was determined by otoluidine method. Serum total cholesterol and triglyceride content were determined by GPOPAP method. Serum superoxide dismutase activity was determined by hydroxylamine method. Serum catalase activity was determined by ammonium molybdate method. The activities of serum glutathione peroxidase， caecal lipase and trypsin were determined by colorimetry. The activity of αamylase in cecum was determined by iodinestarch colorimetry.[Result]There was no significant difference in the content of triglyceride and the activities of catalase， total superoxide dismutase， trypsin and αamylase in each body weight group， while there was no correlation between these biochemical index values and the body weight either. There was no significant difference between total cholesterol content and lipase activity in each body weight group， but there was extremely significant positive correlation （P<0.01） or significant negative correlation （P<0.05） with body weight， respectively. The activity of glutathione peroxidase was significantly different （P<0.01） in each group and negatively correlated with body weight. [Conclusion] The results of this study had a certain guiding effect on the reasonable proportion of nutrient ingredients and the timely addition of exogenous digestive enzymes in the feed of Ningdu Huang chicken .

Key words Ningdu Huang chicken;Body weight;Immunoactive substance;Digestive enzymes;Correlation

宁都黄鸡原名为“宁都三黄鸡”，其肉质鲜美，畅销于沿海地区，是重要的地方家禽养殖品种，也是我国小型优质肉鸡的地方品种资源[1-2]。Ekunseitan 等[3]研究平菇对肉仔鸡胴体、血清代谢物和肌肉脂蛋白含量的影响，结果表明血液生化指标在一定程度上反映了营养价值水平。……