刘琴　李逸潇　张涛静　龚燕冰　周晖　谢培凤

[摘要] 目的 觀察中药复方制剂糖络宁对糖尿病大鼠背根神经节（DRG）线粒体呼吸链氧化呼吸功能、超氧阴离子产生量及膜电位水平的影响。 方法 选取SPF级雄性8周SD大鼠80只，随机选择14只为正常组，其余66只大鼠采用链脲佐菌素制作糖尿病大鼠模型；其中61只造模成功大鼠采用随机数字表法分为模型组19只、西药组（α-硫辛酸组）21只、中药组（糖络宁组）21只，共给药8周；给药结束后，检测线粒体呼吸链氧化呼吸功能、超氧阴离子产生量及膜电位水平。 结果 与正常组比较，模型组大鼠DRG线粒体呼吸功能明显下降（P < 0.01）；与模型组比较，西药组大鼠DRG线粒体呼吸功能明显改善（P < 0.01）；与模型组比较，中药组大鼠DRG线粒体呼吸功能未见明显改善（P > 0.05）。与正常组比较，模型组大鼠DRG线粒体超氧阴离子明显升高（P < 0.01）；与模型组比较，中药组、西药组大鼠DRG线粒体超氧阴离子水平明显降低（P < 0.01）。与正常组比较，模型组大鼠线粒体膜电位明显下降（P < 0.01）；与模型组比较，中药组、西药组大鼠线粒体膜电位明显改善（P < 0.01）。 结论 糖络宁不能改善糖尿病大鼠DRG线粒体氧化呼吸功能，可抑制糖尿病大鼠DRG线粒体超氧阴离子的产生，改善线粒体膜电位水平，这可能是糖络宁治疗糖尿病周围神经病变的作用机制。

[关键词] 糖络宁；糖尿病大鼠；氧化呼吸功能；超氧阴离子；线粒体；膜电位

[中图分类号] R587.1 [文献标识码] A [文章编号] 1673-7210（2018）09（b）-0004-05

[Abstract] Objective To observe the effect of Chinese herbal compound Tang-luo-ning on oxidative respiratory function of the mitochondrial respiratory chain， superoxide anion production and membrane potential in diabetic rat dorsal root ganglion （DRG）. Methods There were 80 SD male rats， 14 of them were randomly selected as the normal group. The rest 66 were used to established diabetic rats model by injected Streptozocin （STZ）. According to the random number table， the 61 rats of the model were divided into model group with 19 rats， Western medicine group （alpha lipoic acid group） with 21 rats and traditional Chinese medicine group （Tang-luo-ning group） with 21 rats. They were given the medicine 8 weeks， then mitochondrial respiratory chain oxidative respiratory function， superoxide anion production and membrane potential levels were measured. Results Compared with the normal group， the mitochondrial respiratory function of the DRG in the model group was significantly decreased （P < 0.01）. Compared with the model group， the mitochondrial respiratory function of the DRG in the Western medicine group was significantly improved （P < 0.01）. Compared with the model group， the Chinese medicine group had no significant improvement in mitochondrial respiratory function in DRG（P > 0.05）. Compared with the normal group， the mitochondrial superoxide anion of the DRG in the model group was significantly increased （P < 0.01）. Compared with the model group， the mitochondrial superoxide anion level of the DRG in the Chinese medicine group and the Western medicine group was significantly reduced （P < 0.1）. Compared with the normal group， the mitochondrial membrane potential of the model group was significantly decreased （P < 0.01）. Compared with the model group， the mitochondrial membrane potential of the Chinese medicine group and the Western medicine group was significantly improved （P < 0.01）. Conclusion Tang-luo-ning inhibits the generation of superoxide anion and ameliorates mitochondrial membrane potential in dorsal root ganglions of diabetic rats. This study provides a potential mechanism underlying protection of Tang-luo-ning on the injury of dorsal root ganglions in diabetic model.

[Key words] Tang-luo-ning； Diabetic rats； Oxidative respiratory function； Superoxide anion； Mitochondria； Membrane potential

糖尿病性周圍神经病变（diabetic peripheral neuropathy，DPN）是糖尿病最常见慢性并发症之一，发病率高，有近半数患者无明显症状[1]。神经病变可累及感觉神经、运动神经及植物神经，产生疼痛、麻木、运动障碍及自主神经功能障碍，影响糖尿病患者的生存质量[2]。氧化应激在DPN的发病中起着关键性的作用，尤其是神经细胞内最重要的产能细胞器——线粒体所导致的氧化应激，被认为是DPN发病的重要机制。高血糖状态下，神经细胞线粒体呼吸链的氧化呼吸功能、超氧阴离子的产生量以及线粒体膜电位均会发生改变，进而导致线粒体中氧化应激反应增强，引发DPN。

中药复方制剂糖络宁治疗DPN效果显著，通过对200例患者进行临床观察，糖络宁的治疗有效率达91.67%[3-5]，显著高于对照组（口服甲钴胺）的62.5%。……