和梦珂 张静 李昕 李凌楠 石静纹 郭健

〔摘要〕 目的 探讨苍附导痰汤对米非司酮诱导多囊卵巢综合征（polycystic ovarian syndrome， PCOS）大鼠卵巢形态及雄激素的影响。方法 60只Wistar大鼠分为正常组、模型组、二甲双胍组和苍附导痰汤高、中、低剂量组。采用皮下注射米非司酮造模并以阴道涂片监测大鼠发情周期，造模成功后各组灌胃干预9 d，处死动物，称量双侧卵巢质量，HE染色观察卵巢形态及卵泡发育情况，放射免疫法测定血清睾酮水平。结果 和正常组比较，模型组大鼠血清睾酮水平、卵巢指数、窦前卵泡及初级卵泡数量明显增加，差异具有统计学意义（P<0.05）；和模型组比较，中药组及二甲双胍组大鼠血清睾酮、窦前卵泡数量、初级卵泡数量都明显降低（P<0.05）。结论 苍附导痰汤作用于米非司酮诱导的PCOS大鼠，可降低血清睾酮水平，使PCOS大鼠窦前卵泡、初级卵泡数量明显减少，改善卵巢多囊样改变。

〔关键词〕 苍附导痰汤；米非司酮；多囊卵巢综合征；卵巢形态；睾酮；窦前卵泡

〔中图分类号〕R285.5；R711.75 〔文献标志码〕A 〔文章编号〕doi：10.3969/j.issn.1674-070X.2018.08.005

〔Abstract〕 Objective To investigate the effect of Cangfu Daotan Decoction on ovarian morphology and androgen in rats with mifepristone-induced polycystic ovarian syndrome（PCOS）. Methods Sixty Wistar rats were divided into normal group， model group， metformin group， high-dose Cangfu Daotan Decoction group， medium-dose Cangfu Daotan Decoction group， and low-dose Cangfu Daotan Decoction group. A rat model of PCOS was established by subcutaneous injection of mifepristone. The oestrous cycle was monitored by vaginal smear. The rats received drugs by gavage for 9 days after successful establishment of the model. The rats were sacrificed， and both ovaries were weighted. HE staining was used to observe the ovarian morphology and follicular development. Radioimmunoassay was used to measure the level of serum testosterone. Results The model group had significantly increased serum testosterone， ovary index， and numbers of preantral and primary follicles than the normal group （P<0.05）. The Cangfu Daotan Decoction groups and the metformin group had significantly reduced serum testosterone and numbers of preantral and primary follicles compared with the model group （P<0.05）. Conclusion For rats with mifepristone-induced PCOS， Cangfu Daotan Decoction can reduce the level of serum testosterone and the numbers of preantral and primary follicles. As a result， the quality of the ovary in rats is reduced and the polycystic changes in the ovary are improved.

〔Keywords〕 Cangfu Daotan Decoction； mifepristone； polycystic ovarian syndrome； ovarian morphology； testosterone； preantral follicle

多囊卵巢綜合征（polycystic ovarian syndrome， PCOS）是妇科常见的内分泌代谢紊乱综合征，发病率占生育年龄妇女的5%～10%。PCOS导致的高度不孕率对患者的身心造成了极大的伤害，但由于PCOS发病原因不明、病理涉及多系统、临床表现多态性，使得PCOS的治疗出现多法多方[1-3]。临床常见PCOS的中医分型有肾阴虚、肾阳虚、痰湿阻滞、肝经湿热、气滞血瘀等[4]。针对痰湿阻滞型PCOS患者，临床应用苍附导痰汤疗效显著[5-9]，然具体的作用机制有待进一步研究，本实验主要探讨苍附导痰汤对米非司酮诱导的PCOS大鼠卵巢形态及雄激素的影响。

1 材料与方法

1.1 实验动物及分组

6周龄Wistar雌性大鼠60只，体质量（200±20） g，清洁级，由北京维通利华实验动物技术有限公司提供，实验动物许可证号：SYXK（京）2014-0010。60只大鼠先随机分出正常组6只，实验组54只。……